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Effects of Dietary Fish Oil, Vitamin E and C Supplementation on DHA Deposition and Shelf-Life in Broiler Chickens

어유, 비타민 E 및 C의 급여가 닭고기의 DHA 축적 및 저장성에 미치는 영향

  • Kang, H.K. (Poultry Science Division, National Institute of Animal Science) ;
  • Kim, S.H. (Poultry Science Division, National Institute of Animal Science) ;
  • Kim, J.H. (Poultry Science Division, National Institute of Animal Science) ;
  • Kang, G.H. (Poultry Science Division, National Institute of Animal Science) ;
  • Yu, D.J. (Poultry Science Division, National Institute of Animal Science) ;
  • Na, J.C. (Poultry Science Division, National Institute of Animal Science) ;
  • Kim, D.W. (Poultry Science Division, National Institute of Animal Science) ;
  • Seo, O.S. (Poultry Science Division, National Institute of Animal Science) ;
  • Kim, G.H. (Kangwon National University) ;
  • Park, B.S. (Kangwon National University)
  • 강환구 (농촌진흥청 축산과학원 가금과) ;
  • 김상호 (농촌진흥청 축산과학원 가금과) ;
  • 김지혁 (농촌진흥청 축산과학원 가금과) ;
  • 강근호 (농촌진흥청 축산과학원 가금과) ;
  • 유동조 (농촌진흥청 축산과학원 가금과) ;
  • 나재천 (농촌진흥청 축산과학원 가금과) ;
  • 김동욱 (농촌진흥청 축산과학원 가금과) ;
  • 서옥석 (농촌진흥청 축산과학원 가금과) ;
  • 김겸헌 (강원대학교 동물생명공학과) ;
  • 박병성 (강원대학교 동물생명공학과)
  • Published : 2007.12.31

Abstract

This study was compared the effect of shelf-life and DHA accumulation in chicken meat from broilers fed experimental diets for two weeks($21{\sim}35$ days) of growers. Two hundred-ten male Ross broilers, 1 day of age, were randomly allocated to seven treatment groups. Experimental diets were assigned to each of the seven groups: control diet containing tallow, T1 with 1.00% fish oil, T2 with 2.00% fish oil, T3 with 2.00% fish oil, 200 ppm vitamin E and 200 ppm vitamin C, T4 with 2.00% fish oil and 200 ppm vitamin C, T5 with 2.00% fish oil and 200 ppm vitamin E, and T6 with 3.00% fish oil. The levels of DHA in chicken meat was the highest in T6, and T1 in breast muscle and thigh muscle with skin was higher than that of $T2{\sim}T5,\;T2{\sim}T5$ in raw chicken meat and wing with skin was about two-fold higher than that of T1. The contents of DHA in chicken meat according to storage days were significantly reduced to 42.30%, 49.38% and 48.51% in T1, T2 and T6, respectively, and this decrease was higher than that of T3, T4 and T5 (p<0.05). Particularly, the rate of reduction of DHA was the lowest in the T3 and T5, which were the lowest in TBARS(thiobarbituric acid reactive substances). TBARS increased in the order of T6, T2 and T1, but reduced in the order of T3, T5 and T4 according to storage days, and there was a significant difference among the treatment groups (p<0.05).

본 연구는 브로일러 후기 2주($21{\sim}35$일령) 동안 실험 사료를 섭취한 닭고기에서 DHA 축적 효과 및 저장성을 비교하였다. 로스종 1일령 병아리 수컷 210수를 7개의 처리구로 구분하여 완전 임의 배치하였다. 실험 처리구는 우지를 함유하는 대조구, 어유 1.00% 첨가구(T1), 어유 2.00% 첨가구(T2), 어유 2.00% + 비타민 E 200 ppm + 비타민 C 200 ppm 첨가구(T3), 어유 2.00% + 비타민 C 200 ppm 첨가구(T4), 어유 2.00% + 비타민 E 200 ppm 첨가구(T5) 그리고 어유 3.00% 첨가구(T6)로 구분하였다. 닭고기 내 DHA 축적량은 모든 부위에서 T6가 가장 높았고, 다리살(닭 껍질포함)과 가슴살 근육에서 T1이 $T2{\sim}T5$ 보다 약간 높았으나, 생통닭과 날개(닭 피부 포함)에서 $T2{\sim}T5$는 T1 보다 약 2배 가까이 높은 축적율을 나타냈다. 저장 일수에 따른 닭고기의 DHA 조성은 T1, T2 및 T6가 42.30%, 49.38%, 48.51% 감소하였으며, 이들은 T3, T4, T5에 비해서 유의적으로 높은 감소를 나타냈다(p<0.05). 특히 TBARS의 값이 가장 낮은 T3와 T5에서 가장 낮은 DHA 감소율을 나타냈다. TBARS는 저장 일수가 지남에 따라서 T6, T2, T1 순서로 증가하였고, T3, T5, T4 순서로 낮았으며, 각 처리구간 통계적인 유의차가 있었다(p<0.05).

Keywords

References

  1. AOAC 1995 Official method of analysis. 16th ed. Association of Official Analytical Chemists. Washington DC. USA. P1-43
  2. Bartov I 1983 Effects of dietary vitamin E on the stability and sensory of turkey meat. Poultry Sci 62:1224-1230 https://doi.org/10.3382/ps.0621224
  3. Bou R, Grimpa S, Guardiola F, Barroeta AC, Codony R 2004 Effect of various fat sources, $\alpha$-tocopheryl acetate, and ascorbic acid supplements on fatty acid composition and $\alpha$-tocopherol content in raw and vacuum-packed, cooked dark chicken meat. Poultry Sci 85:1472-1481
  4. Bou R, Grimpa S, Guardiola F, Barroeta AC, Codony R 2006 Effects of various fat sources, $\alpha$-tocopheryl acetate, and ascorbic acid supplements on fatty acid composition and $\alpha$-tocopherol content in raw and vacuum-packed, cooked dark chicken meat. Poultry Sci 85:1472-1481 https://doi.org/10.1093/ps/85.8.1472
  5. Bou R, Guardiola F, Tres A, Barroeta AC, Codony R 2004 Effect of dietary fish oil, $\alpha$-tocopheryl acetate, and zinc supplementation on the composition and consumer acceptability of chicken meat. Poultry Sci 83:282-292 https://doi.org/10.1093/ps/83.2.282
  6. Bou RF, Guardiola A, Grau S, Munich A, Barroeta A, Codony R 2001 Influence of dietary fat source, $\alpha$-tocopherol, and ascorbic acid supplementation on sensory quality of dark chicken meat. Poultry Sci 80:800-807 https://doi.org/10.1093/ps/80.6.800
  7. Brown AJ, Roberts DCK, Pritchard JE, Truswell AS 1990 A mixed australian fish diet and fish oil supplementation: Impact on the plasma lipid profile of healthy men. Am J Clin Nutr 52:825-833 https://doi.org/10.1093/ajcn/52.5.825
  8. Burdge GC, Jones AE, Wootton SA 2002 Eicospentaenoic and docosahexaenoic acids are the principal products of $\alpha$-linolenic acid metabolism in young men. Br J Nutr 88: 355-363 https://doi.org/10.1079/BJN2002662
  9. Burge JA, Aust JD 1978 Microsomal lipid peroxidation. Methods Enzymol. 52: 302-308 https://doi.org/10.1016/S0076-6879(78)52032-6
  10. Connor WE 2000 Importance of n-3 fatty acids in health and disease. Am J Clin Nutr 71:171S-175S https://doi.org/10.1093/ajcn/71.1.171S
  11. Farrell DJ, Gibson RA 1990 Manipulation of the composition of lipid in egg and poultry meat. In: Smith WC, ed. Proceeding of the inaugural Massey pig and poultry symposium. Palmerston North, New Zealand: Massey University. 164-179
  12. Folch J, Lees M, Sloane-Stanletys GH 1957 A simple method for isolation and purification of total lipids from animal tissues. J Bio Chem 226:497-507
  13. Grau A, Guardiola F, Grimpa S, Barroeta C, Codony R 2001 Oxidative stability of dark chicken meat through frozen storage: Influence of dietary fat and $\alpha$-tocopherol and ascorbic acid supplementation. Poultry Sci 80:1630-1642 https://doi.org/10.1093/ps/80.11.1630
  14. Gray JI, Pearson AM 1987 Rancidity and warm-over flavor. Adv Meat Res 3:221-232
  15. Hargis PS, van Elswyk ME 1993 Manipulating the fatty acid composition of poultry meat and eggs for the health conscious consumer. World's Poultry Sci J 49:251-264 https://doi.org/10.1079/WPS19930023
  16. Houben JH 1998 Effect of the dietary supplementation with vitamin E on colour stability and lipid oxidation in packaged minced pork. Meat Sci 48:265-273 https://doi.org/10.1016/S0309-1740(97)00103-4
  17. Howe PR, Downing JA, Grenyer BF, Grigonis-Deane EM, Bryden WL 2002 Tuna fish meal as a source of DHA for n-3 PUFA enrichment of pork, chicken, and egg. Lipids 37: 1067-1076 https://doi.org/10.1007/s11745-002-1002-3
  18. Jensen C, Lauridsen C, Bertelsen G 1998 Dietary vitamin E: Quality and storage stability of pork and poultry. Trends Food Sci Technol 9:62-72 https://doi.org/10.1016/S0924-2244(98)00004-1
  19. Kinsella JE, Lokesh B, Stone RA 1990 Dietary n-3 polyunsaturated fatty acid ameloration of cardiovascular disease. J Food Sci 60:1009-1012 https://doi.org/10.1111/j.1365-2621.1995.tb06281.x
  20. Leskanich CO, Noble RC 1997 Manipulation of the n-3polyunsaturated fatty acid composition of avain eggs and meat. World's Poultry Sci 53:155-183 https://doi.org/10.1079/WPS19970015
  21. Mantzioris E, James MJ, Gibson RA, Cleland LG 1994 Dietary substitution with an $\alpha$-linolenic acid-rich vegetable oil increase eicosapentaenoic acid concentration in tissues. Am J Clin Nutr 59:1304-1309 https://doi.org/10.1093/ajcn/59.6.1304
  22. Maraschiello C, Sarraga C, Regueior, G 1999 Glutathione peroxidase activity, TBARS, and $\alpha$-tocopherol in meat from chickens fed different diets. J Agric Food Chem 47:867-972 https://doi.org/10.1021/jf980824o
  23. Morrison WR, Smith LM 1967 Preparation of fatty acid methylesters and dimethylacetals from lipid with boron fluoride methanol. J Lipid Res 5:600-608
  24. Nash DM, Hamilton RMG, Hulan HW 1995 The effect of dietary herring meal on the omega-3 fatty acid content of plasma and egg yolk lipids of laying hens. Can J Anim Sci 75:247-253 https://doi.org/10.4141/cjas95-036
  25. National Research Council 1994 Nutrients requirements of poultry. 9th rev. National Academy Press, Washington DC
  26. Pardue SL, Thaxton JP 1986 Ascorbic acid in poultry: A review. World's Poultry Sci J 42:107-123 https://doi.org/10.1079/WPS19860009
  27. Pearson AM, Gray JI, Wolzak AM, Horenstein NA 1983 Safety implications of oxidized lipids in muscle foods. Food Technol 37:121-129
  28. Peter RC 1998 $\omega$-3 Enriched pork. World Rev Nutr Diet Basel Karger. 83:132-143 https://doi.org/10.1159/000059658
  29. SAS Institute 2000 $SAS^{\circR}$ User's guide: Statistics. Version 8 edition SAS Institute Inc., Cary, NC, USA
  30. Sheehy PJ, Morrissey PA, Flynn A 1993 Influence of heated vegetable oils and $\alpha$-tocopheryl acetate supplementation on $\alpha$-tocopherol, fatty acids and lipid peroxidation in chicken muscle. Br Poultry Sci 34:367-381 https://doi.org/10.1080/00071669308417592
  31. Sheldon BW 1984 Effect of dietary tocopherol on the oxidative stability of turkey meat. Poultry Sci 63:673-681 https://doi.org/10.3382/ps.0630673
  32. Simopoulos AP 2000 Human requirement for n-3 polyunsa turated fatty acids. Poultry Sci 79:961-970 https://doi.org/10.1093/ps/79.7.961
  33. Siscovick DS, Raghunathan TE, King I, Weinmann S, Wicklund KG, Albright J, Bovbierg V, Arbogast P, Smith H, Kushi LH, Cobb LA, Copass MK, Psaty BM, Lemaitre R, Retzlaff B, Childs M, Knopp RH 1995 Dietary intake and cell membrane levels of long chain fatty acids and the risk of primary cardiac arrest. J Am Med Assoic 274:1363-1367 https://doi.org/10.1001/jama.274.17.1363
  34. Surai PF, Sparks HC 2000 Tissue-specific fatty acid and $\alpha$-tocopherol profiles in male chickens depending on dietary tuna oil and vitamin E provision. Poultry Sci 79:1132-1142 https://doi.org/10.1093/ps/79.8.1132
  35. Uauy R, Valenzuela A 2000 Marine oils: The health benefits of n-3 fatty acids. Nutrition 16:680-684 https://doi.org/10.1016/S0899-9007(00)00326-9
  36. Whitehead CC, Keller T 2003 An update on ascorbic acid in poultry. World's Poultry Sci J 59:161-184 https://doi.org/10.1079/WPS20030010
  37. Young JF, Stagsted J, Jensen SK, Karlsson AH, Henckel P 2003 Ascorbic acid, $\alpha$-tocopherol, and oregano supplements reduce stress-induced deterioration of chicken meat quality. Poultry Sci 82:1343-1351 https://doi.org/10.1093/ps/82.8.1343

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