Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
권호 표지

Allomyrina Dichotoma Larva 추출물이 췌장 ${\beta}$-세포에서 streptozotocin에 의해 유도된 산화적 손상에 대한 보호효과

Allomyrina Dichotoma Larva Extracts Protect Streptozotocin-induced Oxidative Cytotoxicity

  • Kim, Deok-Song (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Huh, Jin (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • You, Guen-Chang (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Chae, Soo-Chul (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Lee, Oh-Sun (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Lee, Hwang-Hee Blaise (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Lee, Jong-Bin (Department of Biology, College of Natural Sciences, Chonnam National University) ;
  • Kim, Jong-Sun (Department of Biology, College of Natural Sciences, Chonnam National University)
  • 발행 : 2007.12.31
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초록

장수풍뎅이 유충(Allomyrina dichotoma larva, ADL)은 중국의 전통 약재로서, 특히 항산화 효과가 우수하여 항당료 제재로 사용되고 있다. 본 연구에서는 이러한 ADL의 추출물을 이용하여 헴스터 췌장의 ${\beta}$-세포(HIT-T15)에서 Streptozotocin에 의해 유발된 산화적 손상에 대한 보호효과 및 그 작용기전을 조사하였다. ADL추출물은 처리농도 의존적으로 Streptozotocin에 의해 유발된 지질과산화 및 세포 내 자유산 소종의 양을 억제함으로서 ${\beta}$-세포의 산화적 스트레스에 의한 손상을 보호하였다. 또한 DNA laddering 방법을 사용하여 Streptozotocin에 의해 유발된 DNA 손상을 조사한 결과, ADL추출물 처리농도에 비례하여 Streptozotocin에 의해 유발된 DNA 손상이 감소하였다. 이러한 산화적 손상의 억제능 관련 작용 기전을 조사하기 위해 DPPH free radical 소거능을 실시하였다. 그 결과 ADL추출물 자체가 DPPH 자유 레디컬 소거능이 있음을 확인하였으며, 또한 플라스미드를 이용한 Single-strand break 방법을 통한 DNA 손상 보호능을 측정한 결과도 $Fe^{3+}$ 및 $H_2O_2$에 의해 유발된 DNA 손상이 ADL추출물 처리농도에 비례하여 감소하였다. 이러한 결과들을 종합하여 볼 때, 장수풍뎅이 유충의 추출물들이 자체의 레디컬 소거능 및 산화적 손상에 의한 DNA손상을 억제함으로써, Streptozotocin에 의해 유발된 산화적 손상을 억제할 수 있을 것이라 사료된다.

키워드

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