Journal of Applied Biological Chemistry

  • 제50권4호
  • /
  • Pages.264-269
  • /
  • 2007
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

Effects of Root Extracts from Angelica gigas and Angelica acutiloba on Inflammatory Mediators in Mouse Macrophages

  • Yoon, Tae-Sook (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Cheon, Myeoung-Sook (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, Do-Yeon (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong-Cheol (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, Hye-Won (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Department of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 발행 : 2007.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Root extracts of Angelica gigas and A. acutiloba have been used traditionally for the treatment of gynecological diseases, as well as anemia, blood stasis, and inflammatory pain, as blood tonics in Oriental medicine. In the present study, we investigated the effects of A. gigas and A. acutiloba on inflammatory mediators in mouse macrophages and compared their activities. Many studies suggest that prostaglandin $E_2$ ($PGE_2$) biosynthesis and nitric oxide (NO) production play important roles in the processes of both inflammation and carcinogenesis. Ethanolic extracts from the roots of both species exhibited significant inhibitory effects on $PGE_2$ generation in lipopolysaccharide-stimulated RAW 264.7 cells. In particular, the extract from A. gigas was more effective than that from A. acutiloba. Although neither inhibited NO generation, the extract from A. acutiloba stimulated NO generation. Our results suggest that the roots of A. gigas might possess more anti-inflammatory and/or cancer chemopreventative activity than that of A. acutiloba due to the suppression of cyclooxygenase-2 (COX2)-mediated $PGE_2$ production. In addition, A. acutiloba might exert anti-tumor activity through an increase in macrophage-produced NO.

키워드

참고문헌

  1. Aktan F (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci 75, 639-653 https://doi.org/10.1016/j.lfs.2003.10.042
  2. Choi SS, Han KJ, Lee HK, Han EJ, and Suh HW (2003) Antinociceptive profiles of crude extract from roots of Angelica gigas NAKAI in various pain models. Biol Pharm Bull 26, 1283-1288 https://doi.org/10.1248/bpb.26.1283
  3. Choi YH, Son KH, Chang HW, Bae K, Kang SS, and Kim HP (2005) New anti-inflammatory formulation containing Synurus deltoides extract. Arch Pharm Res 28, 848- 853 https://doi.org/10.1007/BF02977352
  4. Farias-Eisner R, Sherman MP, Aeberhard E, and Chaudhuri G (1994) Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc Natl Acad Sci USA 91, 9407-9411
  5. Gallo O, Masini E, Morbidelli L, Franchi A, Fini-Storchi I, Vergari WA, and Ziche M (1998) Role of nitric oxide in angiogenesis and tumor progression in head and neck cancer. J Natl Cancer Inst 90, 587-596 https://doi.org/10.1093/jnci/90.8.587
  6. Gorelik L, Bar-Dagan Y, and Mokyr MB (1996) Insight into the mechanism(s) through which TNF promotes the generation of T cell-mediated antitumor cytotoxicity by tumor bearer splenic cells. J Immunol 156, 4298-4308
  7. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, and Tannenbaum SR (1982) Analysis of nitrate, and [15N]nitrate in biological fluids. Anal Biochem 126, 131-138 https://doi.org/10.1016/0003-2697(82)90118-X
  8. Hakim I, Levy S, and Levy R (1996) A nine-amino acid peptide from IL-1beta augments antitumor immune responses induced by protein and DNA vaccines. J Immunol 157, 5503-5511
  9. Ham M.-S, Kim S-S, Hong J-S, Lee J-H, Chung E-K, Park Y-S, and Lee H-Y (1996) Screening and comparison of active substances of Angelica gigas Nakai produced in Kangwon and Angelica cautiloba Kitagawa produced in Japan. Kor J Appl Microbiol Biotechnol 24, 624-629
  10. Hatano R, Takano F, Fushiya S, Michimata M, Tanaka T, Kazama I, Suzuki M, and Matsubara M (2004) Watersoluble extracts from Angelica acutiloba Kitagawa enhance hematopoiesis by activating immature erythroid cells in mice with 5-fluorouracil-induced anemia. Exp Hematol 32, 918-924 https://doi.org/10.1016/j.exphem.2004.07.003
  11. Kang SA, Jang K-H, Lee JE, Ahn D-K, and Park SK (2003) Differences of hematopoietic effects of Angelica gigas, A. sinensis and A. acutiloba extract on cyclophosphamide- induced anemic rats. Korean J Food Sci Technol 35, 1204-1208
  12. Kumazawa Y, Mizunoe K, and Otsuka Y (1982) Immunostimulating polysaccharide separated from hot water extract of Angelica acutiloba Kitagawa (Yamato tohki). Immunology 47, 75-83
  13. Kumazawa Y, Nakatsuru Y, Fujisawa H, Nishimura C, Mizunoe K, Otsuka Y, and Nomoto K (1985) Lymphocyte activation by a polysaccharide fraction separated from hot water extracts of Angelica acutiloba Kitagawa. J Pharmacobiodyn 8, 417-424 https://doi.org/10.1248/bpb1978.8.417
  14. Lasek W, Feleszko W, Golab J, Stoklosa T, Marczak M, Dabrowska A, Malejczyk M, and Jakobisiak M (1997) Antitumor effects of the combination immunotherapy with interleukin-12 and tumor necrosis factor alpha in mice. Cancer Immunol Immunother 45, 100-108 https://doi.org/10.1007/s002620050408
  15. Lee S, Lee YS, Jung SH, Shin KH, Kim BK, and Kang SS (2003a) Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch Pharm Res 26, 727- 730 https://doi.org/10.1007/BF02976682
  16. Lee S, Shin DS, Kim JS, Oh KB, and Kang SS (2003b) Antibacterial coumarins from Angelica gigas roots. Arch Pharm Res 26, 449-452 https://doi.org/10.1007/BF02976860
  17. Lee YY, Lee S, Jin JL, and Yun-Choi HS (2003c) Platelet anti-aggregatory effects of coumarins from the roots of Angelica genuflexa and A. gigas. Arch Pharm Res 26, 723-726 https://doi.org/10.1007/BF02976681
  18. Leiper J and Vallance P (1999) Biological significance of endogenous methylarginines that inhibit nitric oxide synthases. Cardiovasc Res 43, 542-548 https://doi.org/10.1016/S0008-6363(99)00162-5
  19. Mordan LJ, Burnett TS, Zhang LX, Tom J, and Cooney RV (1993) Inhibitors of endogenous nitrogen oxide formation block the promotion of neoplastic transformation in C3H 10T1/2 fibroblasts. Carcinogenesis 14, 1555-1559 https://doi.org/10.1093/carcin/14.8.1555
  20. Ohshima H and Bartsch H (1994) Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res 305, 253-264 https://doi.org/10.1016/0027-5107(94)90245-3
  21. Oshima M, Dinchuk JE, Kargman SL, Oshima H, Hancock B, Kwong E, Trzaskos JM, Evans JF, and Taketo MM (1996) Suppression of intestinal polyposis in Apc delta716 knockout mice by inhibition of cyclooxygenase 2 (COX-2). Cell 87, 803-809 https://doi.org/10.1016/S0092-8674(00)81988-1
  22. Palmer RM, Ashton DS, and Moncada S (1988) Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 333, 664-666 https://doi.org/10.1038/333664a0
  23. Radomski MW, Jenkins DC, Holmes L, and Moncada S (1991) Human colorectal adenocarcinoma cells: differential nitric oxide synthesis determines their ability to aggregate platelets. Cancer Res 51, 6073-6078
  24. Sarker SD and Nahar L (2004) Natural medicine: the genus Angelica. Curr Med Chem 11, 1479-1500 https://doi.org/10.2174/0929867043365189
  25. Schmidt HH and Walter U (1994) NO at work. Cell 78, 919-925 https://doi.org/10.1016/0092-8674(94)90267-4
  26. Simon LS (1999) Role and regulation of cyclooxygenase-2 during inflammation. Am J Med 106, 37S-42S https://doi.org/10.1016/S0002-9343(99)00115-1
  27. Song S-H, Seo B-I, Kim H-K, and Park J-H (2004) The effects of Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix extract on hydrocortisone acetate-induced model of blood stasis. Kor J Herbology 19, 13-21
  28. Subbaramaiah K, Telang N, Ramonetti JT, Araki R, DeVito B, Weksler BB, and Dannenberg AJ (1996) Transcription of cyclooxygenase-2 is enhanced in transformed mammary epithelial cells. Cancer Res 56, 4424-4429
  29. Tanaka S, Ikeshiro Y, Tabata M, and Konoshima M (1977) Anti-nociceptive substances from the roots of Angelica acutiloba. Arzneimittelforschung 27, 2039-2045
  30. Thomsen LL, Miles DW, Happerfield L, Bobrow LG, Knowles RG, and Moncada S (1995) Nitric oxide synthase activity in human breast cancer. Br J Cancer 72, 41-44 https://doi.org/10.1038/bjc.1995.274
  31. Vane JR, Bakhle YS, and Botting RM (1998) Cyclooxygenases 1 and 2. Annu Rev Pharmacol Toxicol 38, 97-120 https://doi.org/10.1146/annurev.pharmtox.38.1.97
  32. Xie K, Huang S, Dong Z, Juang SH, Gutman M, Xie QW, Nathan C, and Fidler IJ (1995) Transfection with the inducible nitric oxide synthase gene suppresses tumorigenicity and abrogates metastasis by K-1735 murine melanoma cells. J Exp Med 181, 1333-1343 https://doi.org/10.1084/jem.181.4.1333
  33. Xie QW, Kashiwabara Y, and Nathan C (1994) Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J Biol Chem 269, 4705-4708
  34. Yamada H, Komiyama K, Kiyohara H, Cyong JC, Hirakawa Y, and Otsuka Y (1990) Structural characterization and antitumor activity of a pectic polysaccharide from the roots of Angelica acutiloba. Planta Med 56, 182-186 https://doi.org/10.1055/s-2006-960921
  35. Yim CY, Bastian NR, Smith JC, Hibbs JB, Jr and Samlowski WE (1993) Macrophage nitric oxide synthesis delays progression of ultraviolet light-induced murine skin cancers. Cancer Res 53, 5507-5511
  36. Yim D, Singh RP, Agarwal C, Lee S, Chi H, and Agarwal R (2005) A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res 65, 1035-1044
  37. Yip I, Pang XP, Berg L, and Hershman JM (1995) Antitumor actions of interferon-gamma and interleukin-1 beta on human papillary thyroid carcinoma cell lines. J Clin Endocrinol Metab 80, 1664-1669 https://doi.org/10.1210/jc.80.5.1664