Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Quantitation of Formate in Plants and Its Enhancement in Response to Environmental Stresses

  • Kim, Jae-Kwang (National Institute of Agricultural Biotechnology) ;
  • Cho, Myoung-Rae (National Institute of Agricultural Biotechnology) ;
  • Baek, Hyung-Jin (National Institute of Agricultural Biotechnology) ;
  • Ryu, Tae-Hun (National Institute of Agricultural Biotechnology) ;
  • Kim, Jung-Bong (National Institute of Agricultural Biotechnology) ;
  • Kim, Jun-Heong (National Institute of Agricultural Biotechnology) ;
  • Kim, Myong-Jo (National Institute of Agricultural Biotechnology) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University) ;
  • Fukusaki, Ei-Ichiro (Department of Biotechnology, Graduate School of Engineering, Osaka University) ;
  • Kobayashi, Akio (Department of Biotechnology, Graduate School of Engineering, Osaka University)
  • Published : 2007.12.31
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Abstract

A solid-phase microextraction and gas chromatography-mass spectrometry utilizing $^{13}C$-formate as an internal standard for the determination of formate was proved to be applicable as a reliable quantitative method in several plants. We were the first to discover that trees contain larger pool sizes of formate than herbs. Our data also showed that the formate level of the leaves increased after the methanol-spraying, suggesting that methanol oxidation could convert formaldehyde into formate. In addition, drought and chilling led to the increase of endogenous formate in Arabidospsis thaliana. These results confirmed that formate is a universal stress signal in plants.

Keywords

References

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