Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Purification and Properties of Intracellular Invertase from Alkalophilic and Thermophilic Bacillus cereus TA-11

  • Yoon, Min-Ho (Department of BioEnvironmental Chemistry, College of Agriculture and Life, Chungnam National University) ;
  • Choi, Woo-Young (Department of BioEnvironmental Chemistry, College of Agriculture and Life, Chungnam National University) ;
  • Kwon, Su-Jin (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Yi, Sung-Hun (Korea Food Research Institute) ;
  • Lee, Dae-Hyung (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • Published : 2007.12.31
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Abstract

An intracellular invertase was purified to homogeneity from the cell extract of an alkalophilic and thermophilic Bacillus sp. TA-11, which was classified as a new species belonging to Bacillus cereus based on chemotaxanomic and phylogenetic analyses. The purified enzyme with a recovery of 26.6% was determined to be a monomeric protein with a molecular weight of 23 kDa by SDS-PAGE and 26 kDa by gel filtration. The maximum enzyme activity was observed at pH 7.0 and $50^{\circ}C$, and the purified enzyme was stable at the pH range of 5.0 to 8.0 and below $60^{\circ}C$. $K_m$ and $V_{max}$ values of the enzyme for sucrose were 370 mM and 3.0 ${\mu}M$ per min, respectively. The enzyme activity was significantly inhibited by bivalent metal ions ($Hg^{2+}$, $Cd^{2+}$ and $Cu^{2+}$) and sugars (glucose and fructose).

Keywords

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