Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Culture Conditions and Freezing Methods on Developmental Competence of Hanwoo Embryos Cultured In Vitro

  • Song, S.H. (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Min, C.S. (GyeongSangNamDo Agriculture Research & Extension Services) ;
  • Son, G.D. (Division of Applied Life Science, Gyeongsang National University) ;
  • Rho, C.W. (GyeongSangNamDo Agriculture Research & Extension Services) ;
  • Kang, Y.S. (GyeongSangNamDo Agriculture Research & Extension Services) ;
  • Park, C.S. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kong, I.K. (Division of Applied Life Science, Gyeongsang National University)
  • Published : 2007.12.31
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Abstract

This study was carried out to examine on developmental competence of Hanwoo embryos cultured in vitro according to culture conditions and freezing methods. The in vitro developmental competence to blastocyst stage at Day 8 of culture in SOF was significantly (p<0.05) higher than that in CR1aa (30.3% vs. 18.4%). The in vitro developmental rate of morula and blastocysts cultured in group culture was significantly (p<0.05) higher than that in individual culture (41.4% and 36.0% vs. 21.1% and 10.5%, respectively). The cell number of Day 8 blastocysts in group culture was significantly (p<0.05) higher than that in the individual culture ($120.1{\pm}12.8\;vs.\;94.1{\pm}12.1$, respectively). The survival rates of frozen-thawed balstocysts that were exposed in 1.5 M ethylene glycol or 1.5 M ethylene glycol containing 0.1 M sucrose were 77.5% and 78.7%, respectively. The survival rates of blastocysts cultured for 48 h in slow freezing and vitrification was not significantly different (73.3 and 74.0%). In conclusion, in vitro developmental competence of bovine embryos was influenced on the culture medium (SOF) and culture method (Group culture). Survival rate of frozen-thawed of bovine embryos was not influenced on freezing solutions and freezing methods.

Keywords

References

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