Applied Microscopy

  • 제37권4호
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  • Pages.209-217
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  • 2007
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

양서류 피부 상처회복과정 중 각질화세포 cytokeratin의 분포

The Temporal and Spatial Expression of the Cytokeratin in Keratinocytes during Cutaneous Wound Healing on the Amphibian(Bombina orientalis)

  • 임도선 (을지대학교 보건과학대학 치위생학과) ;
  • 정순정 (조선대학교 치과대학 구강조직학교실) ;
  • 문명진 (단국대학교 첨단과학대학 생물학과) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실)
  • Lim, Do-Seun (Department of Dental Hygiene, College of Health Science, Eulji University) ;
  • Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Moon, Myung-Jin (Department of Biological Science & Basic Science, Dankook University) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
  • 발행 : 2007.12.31
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초록

상처회복은 염증, 재상피화 그리고 기질의 재형성등이 관여하는 복잡한 과정이다. 이중 재상피화에 관여하는 각질화세포의 이동경로를 분석하기 위하여 무당개구리 피부 상처유도 후 투과전자현미경과 cytokeratin에 대한 조직면역화학법을 이용하였다. 정상조직의 cytokeratin발현은 기저층의 세포들과 선상피에서 확인되었다. 상처 유도후 3시간 조직에서, 기저세포층에서 강한 반응이 관찰되었고, 1일과 2일 사이에서는 재생되는 각질화세포에서 강한 면역반응이 확인되었다. 상처반응 중기인 7일부터 10일 사이에서도 재생된 세포의 기저층세포에서 강한 반응이 일어났다. 19일경과의 조직에서는 기저층과 유극층의 세포들에서 cytokeratin의 발현이 증가하였다. 따라서, 재상피화에 관여하는 각질화세포는 기저층의 세포로부터 시작하여 상처부위로 이동하여 과립층과 유극층으로 분화됨으로서 재상피가 진행되었다.

The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog(Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer fells and the keratinocytes migrate into basal and middle of the wound area.

키워드

참고문헌

  1. Betz P, Nerlich A, Tubel J, Penning R, Eisenmenger W: The time-dependent expression of keratins 5 and 13 during the reepithelialization of human skin wounds. Int J Leg Med 105 : 229-232, 1993 https://doi.org/10.1007/BF01642799
  2. Cooper EL, Roch P: Earthworm leukocyte interactions during early stages of graft rejection. J Exp Zool 232(1) : 67-72, 1984 https://doi.org/10.1002/jez.1402320109
  3. Cornec JP, Coulomb-Gay R: Demonstration of a regenerating activity in the rhynchobdellid leech Helobdella stagnalis. Arch Anat Microsc Morphol Exp 71(4) : 227-239, 1982
  4. Fuchs E, Green H: Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19 : 1033-1042,1980 https://doi.org/10.1016/0092-8674(80)90094-X
  5. Gibbins JR: Epithelial migration in organ culture. A morphological and time lapse cinematographic analysis of migrating stratified squamous epithelium. Pathology 10 : 207-218, 1978 https://doi.org/10.3109/00313027809063503
  6. Guesdon YL, Ternynck T, Avrameas S: The use of avidinbiotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27 : 1131-1139, 1979 https://doi.org/10.1177/27.8.90074
  7. Huguet G, Molinas M: Changes in epithelial cells in Hirudo medicinalis during wound healing. J Invertebr Pathol 59(1) : 11-17, 1992 https://doi.org/10.1016/0022-2011(92)90105-D
  8. Jensen PKA, Bolund L: Low $Ca^{++}$ stripping of differentiating cell layers in human epidermal cultures: an in vitro model of epidermal regeneration. Exp Cell Res 175-: 63-73, 1988 https://doi.org/10.1016/0014-4827(88)90255-8
  9. Jeong MJ, Moon MJ: Early healing responses of the skin wounds in the Korean fire-bellied toad, Bombina orientalis. Kor J Electr Micr 28(3) : 329-343, 1998
  10. Jeong MJ, Moon MJ: Remodeling of epidermis during skin wound healin in Bombina orientalis. Kor J Electr Micr 29(2) : 201-209, 1999
  11. LeGore RS, Sparks AK: Repair of body wall incision in the rhynchobdellid leech Piscicola salmositica. J Invertebr Pathol 18(1): 40-45, 1971 https://doi.org/10.1016/0022-2011(91)90006-C
  12. LeGore RS, Sparks AK: Repair of body wall burns in the rhynchobdellid leech Piscicola salmositica. J Invertebr Pathol 22(2) : 298-299, 1973 https://doi.org/10.1016/0022-2011(73)90151-1
  13. Mansbridge JN, Knapp AM: Changes in keratinocyte maturation during wound healing. J Invest Dermatol 89 : 253-263, 1987 https://doi.org/10.1111/1523-1747.ep12471216
  14. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R: The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31 : 1124, 1982
  15. Moll I, Houdek P, Schmidt DL, Moll R: Characterization of epidermal wound healing in a human skin organ culture model: acceleration by transplanted keratinocytes. J Invest Dermatol 111 : 251-258, 1998 https://doi.org/10.1046/j.1523-1747.1998.00265.x
  16. Ortonne JP, Loning T, Schmitt D, Thivolet J: Immunomorphological and ultrastructural aspects of keratinocyte migration in epidermal wound healing. Virchows Arch (Pathol Anat) 392: 217-230, 1981 https://doi.org/10.1007/BF00430822
  17. Read J, Watt FM: A model for in vitro studies of epidermal homeostasis: proliferation and involucrin synthesis by cultured human keratinocytes during recovery after stripping off the suprabasal layers. J Invest Dermatol 90 : 739-743, 1988 https://doi.org/10.1111/1523-1747.ep12560940
  18. Schlegel R, Banks-Schlegel S, Pinkus GS: Immunohisto-chemical localization of keratin in normal human tissues. Lab Invest 42 : 91-96, 1980
  19. Sciubba JJ: Regeneration of the basal lamina complex during epithelial wound healing. J Periodont Res 12 : 204-217, 1977 https://doi.org/10.1111/j.1600-0765.1977.tb00123.x
  20. Stenn KS: Keratinocyte adherence. J Invest Dermatol 86(2) : 214-218, 1986
  21. Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95 : 580-588, 1982 https://doi.org/10.1083/jcb.95.2.580