Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Enhanced Tolerance to Oxidative Stress of Transgenic Potato (cv. Superior) Plants Expressing Both SOD and APX in Chloroplasts

SOD와 APX를 동시에 엽록체에 발현시킨 형질전환 감자 (cv. Superior)의 산화스트레스 내성 증가

  • Tang, Li (Environmental Biotechnology Research Center) ;
  • Kwon, Suk-Yoon (Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Myoung-Duck (Environmental Biotechnology Research Center) ;
  • Kim, Jin-Seog (Biofunction Research Team, Bioorganic Science Division, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Kwak, Sang-Soo (Environmental Biotechnology Research Center) ;
  • Lee, Haeng-Soon (Environmental Biotechnology Research Center)
  • 탕리 (한국생명공학연구원 환경생명공학연구센터) ;
  • 권석윤 (식물유전체연구센터) ;
  • 김명덕 (한국생명공학연구원 환경생명공학연구센터) ;
  • 김진석 (한국화학연구원 생물기능연구팀) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구센터) ;
  • 이행순 (한국생명공학연구원 환경생명공학연구센터)
  • Published : 2007.12.31
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Abstract

Oxidative stress is a major damaging factor for plants exposed to environmental stresses. Previously, we have generated transgenic potato (cv. Superior) plants expressing both CuZnSOD and APX genes in chloroplast under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants) and selected the transgenic potato plant lines with tolerance against methyl viologen (MV)-mediated oxidative stress. When leaf discs of SSA plants were subjected to $3{\mu}M$ methyl viologen (MV), they showed approximately 40% less damage than non-transgenic (NT) plants. SSA plantlets were treated with $0.3{\mu}M$ MV stress, SSA plants also exhibited reduced damage in root growth. When 350 MV was sprayed onto the whole plants, SSA plants showed a significant reduction in visible damage, which was approximately 75% less damage than leaves of NT plants. These plants will be used for further analysis of tolerance to environmental stresses, such as high temperature and salt stress. These results suggest that transgenic potato (cv. Superior) plants would be a useful plant crop for commercial cultivation under unfavorable growth conditions.

산화스트레스 유도성 SWPA2 프로모터 조절하에 항산화 효소 SOD와 APX 유전자를 동시에 엽록체에 발현시킨 형질전환 감자 (품종 수미)를 대상으로 methyl viologen (MV) 처리에 의해 유도되는 산화스트레스 내성을 잎절편체, 소식물체 및 식물체 수준에서 조사하였다. 잎 절편에 $3{\mu}M$ MV를 처리하였을 때 SSA 식물체의 잎절편체는 비형질전환 (NT) 식물체에 비해 40% 정도 상해를 적게 받았다. 소식물체 수준에서 MV에 의한 산화스트레스 내성을 조사하기 위하여 SSA감자 shoot을 $0.3{\mu}M$ MV 첨가 배지에 배양하였을 때 뿌리의 생장에서 내성이 나타났다. 또한 온실에서 4주 생장한 식물체에 $350{\mu}M$ MV를 처리하였을 경우에도 SSA 식물체는 NT 식물체에 비해 약 75% 손상을 적게 입은 것으로 나타내었다. 추후 SSA 식물체를 이용하여 건조, 고온 등의 복합재해에 내성을 분석이 진행되어야 할 것이며 그 결과 복합스트레스 내성 감자 품종 (수미)을 개발할 수 있을 것으로 기대한다.

Keywords

References

  1. Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107: 1049-1054 https://doi.org/10.1104/pp.107.4.1049
  2. Arakawa T, Chong DKX, Merritt JL, Langridge WHR (1997) Expression of chorera toxin B sublunit oligomers in transgenic potato plants. Transgenic Res 6: 403-413 https://doi.org/10.1023/A:1018487401810
  3. Asada K (1999) The water-water cycle in chloroplasts: Scavenging of active oxygen an dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50: 601-639 https://doi.org/10.1146/annurev.arplant.50.1.601
  4. Choi KH, Jeon JH, Kim HS, Joung YH, Cho SJ, Lim YP, Joung H (1996) Development of herbicide-resistant transgenic potato. Korean J Plant Tissue Culture 23: 161-165
  5. Chong DK, Langridge WH (2000) Expression of full-length bioactive antimicrobial human lactoferrin in potato plants. Transgenic Res 9: 71-78 https://doi.org/10.1023/A:1008977630179
  6. Foyer CH, Descourvieres P, Kunert KJ (1994) Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell Environ 17: 507-523 https://doi.org/10.1111/j.1365-3040.1994.tb00146.x
  7. Inze D, Van Montagu M (1995) Oxidative stress in plants. Curr Opin Biotechnol 6: 166-172
  8. Kim KY, Kwon SY, Lee HS, Hur YK, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweet potato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol Biol 51: 831-838 https://doi.org/10.1023/A:1023045218815
  9. Kwon SY, Jeong YJ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl violoqen-rnediated oxidative stress. Plant Cell Environ 25: 873-882 https://doi.org/10.1046/j.1365-3040.2002.00870.x
  10. Kwon SY, Choi SM, Ahn YO, Lee HS, Lee HB, Park YM, Kwak SS (2003) Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene. J Plant Physiol 160: 347-353 https://doi.org/10.1078/0176-1617-00926
  11. Lim S, Kim YH, Kim SH, Kwon SY, Lee HS, Kim JS, Cho KY, Paek KY, Kwak SS (2007) Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling. Mol Breeding 19: 227-239 https://doi.org/10.1007/s11032-006-9051-0
  12. McKersie BD, Bowlwy SR, Harjanto E, LePrince O (1996) Water-deficit tolerance and field performance of transgenic alfalfa overexpression superoxide dismutase. Plant Physiol 111: 1177-1181 https://doi.org/10.1104/pp.111.4.1177
  13. Moon HJ, Lee BY, Choi G, Shin DJ, Prasad T, Lee OS, Kwak SS, Kim DH, Nam JS, Bahk JD, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two ocidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci 100: 358-363
  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Oberschall A, Deak M, Torok K, Saa L, Vass I, Kovacs I, Feher A, Dudits D, Horvath GV (2000) A novel aldose/aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses. Plant J 24: 437-446 https://doi.org/10.1046/j.1365-313x.2000.00885.x
  16. Roxas VP, Smith RK, Jr Allen ER, Allen RD (1997) Overexpression of glutathione-S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress. Nat Biotechnol 15: 988-991 https://doi.org/10.1038/nbt1097-988
  17. Slooten L, Capiau K, Van Camp W, Van Montagu M, Sybesma C, Inze D (1995) Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplants. Plant Physiol 107: 737-750 https://doi.org/10.1104/pp.107.3.737
  18. Tang Li (2005) Development and characterization of transgenic potato plants with enhanced tolerance to environmental stress. PhD thesis, Chungnam National University, Daejeon
  19. Tang Li, Kwon SY, Kim SH, Kim JS, Choi JS, Cho KY, Sung CK, Kwak SS, Lee HS (2006) Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature. Plant Cell Rep 25: 1380-1386 https://doi.org/10.1007/s00299-006-0199-1
  20. Tang Li, Kwon SY, Kwak SS, Sung CK, Lee HS (2003) Susceptibility of two cultivars to various environmental stresses. Korean J Plant Biotechnol 30: 405-410 https://doi.org/10.5010/JPB.2003.30.4.405
  21. Tang Li, Kwon SY, Kwak SS, Sung CK, Lee HS (2004a) Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. Korean J Plant Biotechnol 31: 109-113 https://doi.org/10.5010/JPB.2004.31.2.109
  22. Tang Li, Kwon SY, Yun DJ, Kwak SS, Lee HS (2004b) Selection of transgenic potato plants expressing NDP kinase 2 gene with enhanced tolerance to oxidative stress. Korean J Plant Biotechnol 31: 191-195 https://doi.org/10.5010/JPB.2004.31.3.191
  23. Tepperman JM, Dunsmuir P (1990) Transformed plants with elevated levels of chloroplastic SOD are not more resistant to superoxide toxicity. Plant Mol Biol 14: 501-511 https://doi.org/10.1007/BF00027496
  24. Van Camp W, Capiau K, Van Montague M, lnze D, Stoolen L (1996) Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol 112: 1703-1714 https://doi.org/10.1104/pp.112.4.1703
  25. Youm JW, Jeon JH, Jung JY, Lee BC, Kang WJ, Kim MS, Kim CJ, Joung H, Kim HS (2002) Production of VP6 gene into potato plants by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes. Korean J Plant Biotech 29: 93-98 https://doi.org/10.5010/JPB.2002.29.2.093
  26. Yun BW, Huh GH, Lee HS, Kwon SY, Jo JK, Kim JS, Cho KY, Kwak SS (2000) Differential resistance to methyl viologen in transgenic tobacco plants that express sweetpotato peroxidases. J Plant Physiol 156: 504-509 https://doi.org/10.1016/S0176-1617(00)80165-0

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