Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Torilis Fructus on Procollagen Biosynthesis and Activity of Matrix Metalloproteinase-I(MMP-1) in Human Dermal Fibroblast

사상자(Torilis Fructus)가 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-I(MMP-1)의 활성에 미치는 영향

  • Koo, Bon-Suk (Health Food Research Group, KT&G Central Research Institute) ;
  • Hwang, Eui-Il (Health Food Research Group, KT&G Central Research Institute) ;
  • So, Seung-Ho (Health Food Research Group, KT&G Central Research Institute) ;
  • Lee, Seong-Kye (Health Food Research Group, KT&G Central Research Institute) ;
  • Han, Gyeong-Ho (Health Food Research Group, KT&G Central Research Institute) ;
  • Kim, Na-Mi (Health Food Research Group, KT&G Central Research Institute)
  • 구본석 (KT&G중앙연구원 건강식품연구소) ;
  • 황의일 (KT&G중앙연구원 건강식품연구소) ;
  • 소승호 (KT&G중앙연구원 건강식품연구소) ;
  • 이성계 (KT&G중앙연구원 건강식품연구소) ;
  • 한경호 (KT&G중앙연구원 건강식품연구소) ;
  • 김나미 (KT&G중앙연구원 건강식품연구소)
  • Published : 2007.12.31
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Abstract

Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase-I(MMP-1) activity. This study was carried out to find out skin wrinkle reducing components in Torilis Fructus. Torilis Fructus were extracted with 70% ethanol and the ethanol extracts were systematically fractionated with n-hexane, ethylacetate, n-butanol and distilled water. Among them, antiwrinkle component from n-hexane fraction was purified by several column chromatographies and HPLC, which identified as torilin by $^1H-NMR,\;^{13}C-NMR$ and ESI-MS. To determine cell viability, collagen biosynthesis and MMP-1 activity, human dermal fibroblast was treated with 1-5 ppm concentrations of Torilis Fructus extract fraction and torilin. Cell viability was showed 84-102% at all group treated with 1-5 ppm. Collagen synthesis was increased in all group, especially torilin-treated group was highest amount. Active forms of MMP-1 were decreased in all group. From these results, we consider that Torilis Fructus have several antiwrinkle components and torilin may be one of the effective components.

Keywords

References

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