Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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The Effects of HAp and BCP Nano Powders Synthesized by Microwave-Assisted Synthesis on the Activation of Osteoblast and Osteoclast

마이크로웨이브법에 의해 제조된 HAp 및 BCP 분말이 뼈모세포 및 파골세포의 활성에 미치는 영향

  • Song, Ho-Yeon (Department of Microbiology School of Medicine, Soonchunhyang University) ;
  • Min, Young-Ki (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Yang, Hun-Mo (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Mang, Joo-Yang (Department of Chemistry Soonchunhyang University)
  • 송호연 (순천향대학교 의과대학 미생물학교실) ;
  • 민영기 (순천향대학교 의과대학 생리학교실) ;
  • 양훈모 (순천향대학교 의과대학 생리학교실) ;
  • 맹주양 (순천향대학교 화학과)
  • Published : 2007.12.31
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Abstract

Hydroxyapatite (HAp) and biphasic calcium phosphate (BCP) nano powders were synthesized using the microwave-assisted synthesis process dependent on pH and microwave irradiation time. The average size of a powder was less than 100 nm in diameter. Through in-vitro cytotoxicity tests by an extract dilution method, the HAp and BCP nano powders have shown to be cytocompatible for L-929 fibroblast cells, osteoblastlike MG-63 cells and osteoclast-like Raw 264.7 cells. The activation of osteoblast was estimated by alkaline phosphatase (ALP) activity. When the HAp and BCP were treated to MG-63 cells, alkaline phosphatase activities increased on day 3, compared with those of the untreated cells. Also, the collagen fibers increased when the HAp and BCP powders suspension were treated to MG-63 cells, compared to those of the untreated cells. Quantitative alizarin red S mineralization assays showed a trend toward increasing mineralization in osteoblast cultured with powder suspension. In conclusion, hydroxyapatite and biphasic calcium phosphate appeared to be a bone graft substitute material with optimal biocompatibility and could be further applied to clinical use as an artificial bone graft substitute.

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