DOI QR코드

DOI QR Code

당뇨유발 흰쥐에 있어서 산화적 스트레스에 대한 함박잎새버섯의 효과

Effects of Hanbag Mushroom(Grifola frondosa) on Oxidative Stress in Diabetic Rats

  • 이순이 (아시아대학교 뷰티.주얼리디자인학과) ;
  • 이창윤 (㈜그린피스) ;
  • 박영철 (대구가톨릭대학교 의생명과학과, 바이오안전성센터) ;
  • 김종봉 (대구가톨릭대학교 의생명과학과, 바이오안전성센터)
  • Lee, Soon-Yi (Dept. of Beauty.jewely design, Asia University) ;
  • Lee, Chang-Yun (Greenpeace Ltd.) ;
  • Park, Yeong-Chul (Dept. of Medicinal Life Science & Center for Bio-Safety, Catholic University of Daegu) ;
  • Kim, Jong-Bong (Dept. of Medicinal Life Science & Center for Bio-Safety, Catholic University of Daegu)
  • 발행 : 2007.11.30

초록

본 연구는 당뇨병으로 인한 산화적 스트레스에 대한 함박잎새버섯분말의 효과를 밝히기 위하여 SD계 흰쥐를 STZ로 당뇨를 유발하여 간 및 신장 조직에서 조사하였다. 또한 당뇨흰쥐에 함박잎새버섯분말 1-2% 첨가하여 6주간 식이하였다. 산화적 스트레스의 지표물질인 LPO를 비롯하여 유발원 XOD 활성도를 측정하였다. 또한 이에 따른 간조직 손상 확인을 위해 혈청 ALT와 AST 활성도를 측정하였다. 특히 함박잎새버섯분말의 항산화적 효능을 위해 이들 지표물질들과 더불어 항산화체계의 중요 요소인 GSH 농도와 GST 활성도를 당뇨군, 당뇨-잎새버섯분말투여군 그리고 정상군에서 측정하였다. 당뇨군은 정상군과 비교하여 LPO 농도를 비롯하여 XOD 활성도가 유의하게 높았다. 특히 이러한 결과로 추정되는 간 조직 손상이 정상군보다 유의하게 높은 ALT 및 AST 활성도가 혈청에서 확인되었다. 그러나 당뇨-잎새버섯분말투여군에서는 LPO 농도, XOD 활성도를 비롯하여 조직손상의 지표인 ALT 및 AST 활성도가 당뇨군보다 유의하게 감소하였다. 항산화물질인 GSH 농도는 당뇨군 및 당뇨-잎새버섯분말투여군 비교에서 유의한 차이가 없었으나 GST 활성도는 당뇨-잎새버섯분말투여군이 당뇨군보다 유의하게 높았다. 따라서 당뇨유발성 산화적 스트레스에 대한 잎새버섯분말의 효능은 GSH 농도 변화보다 GST 활성도를 증가시키고 또한 산화적 스트레스의 유발원인 XOD 활성도 감소의 유도를 통해 이루어지는 것으로 추정된다. 결론적으로 당뇨는 산화적 스트레스를 증가시키며 조직손상을 유발한다. 그러나 함박잎새버섯분말은 항산화물질 및 효소계의 활성도를 증가시켜 당뇨유발-산화적 스트레스 감소를 유도하여 조직 손상을 감소시키는 것이 확인되었다.

This research was carried out to investigate the effects of Hambag mushroom on the oxidative stress in diabetic rats, Sprague-Dawley. The diabetic rats induced by streptozotocin were fed with hambag mushroom-powder(G. frondosa) for 6 weeks. For the level of oxidative stress in liver and pancreas tissues, it was studied by measuring LPO (lipid oxide) level as an indicator of lipid peroxidation, XOD(xanthine oxidase) as one of important sources for free radicals and the levels of GSH and GST as anti-oxidant systems. Also, as an indicator of liver damaged by oxidative stress, the activities of serum ALT and AST were measured. It was observed that the levels of ALT, AST, LPO and XOD were higher by about two times in both tissues from diabetic rats than in those from control rats. This indicates that the oxidative stress induced by diabetes caused the tissues damages. However, these levels were decreased in the tissues from rats with hambag mushroom-powder. Futhermore, the activity of GST were higher in both tissues from diabetic rats fed with hambag mushroom-powder than in those from diabetic rats. Thus, it is considered that the hambag mushroom-powder decreases the level of oxidative stress by increasing activity of anti-oxidant system such as GSH and GST. It is suggested that the hambag mushroom-powder can be useful for preventing the tissues damaged by diabetes-induced oxidative stress.

키워드

참고문헌

  1. Adachi, Y., M. Okazaki and N. Ohno. 1994. Enhancement of cytokine production by macrophages stimulated with (1 $\rightarrow$3)-beta-D-glucan, grifolan(Grn), isolated from Grifola frondosa. Biol. Pharm. Bull. Dec. 17(12), 1554-1560. https://doi.org/10.1248/bpb.17.1554
  2. Baynes, J. M. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40, 405-412. https://doi.org/10.2337/diabetes.40.4.405
  3. Cho, S. H., K. M. Yang, B. S. Bae, S. N. Im and R. N. Yu. 1998. Effect of sea tangle intake on cytokine production in macrophage from normal and diabetic mice. J. Kor. Soc. Food. Sci. Nutr. 27(5), 952-959.
  4. Ellman, G. L. 1959. Tissue sulfhydryl group. ABB. 82, 70-77.
  5. Habig, W. H., M. J. Pabist and W. B. Jakoby. 1974. Glutathione S-transferase: The frist enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139.
  6. Ham, Y. K. and S. W. Kim. 2004. Protective effects of plant extract of plant extract on the hepatocytes of rat treated with carbon tetrachloride. J. Kor. Sor. Food. Sci. Nutr. 33(8), 1246-1251. https://doi.org/10.3746/jkfn.2004.33.8.1246
  7. Jenkins, A. J., R. L. Klein, C. N. Chassereau et al. 1996. LDL from patients with well-controlled IDDM is not more susceptible to in vitro oxidation. Diabetes 45, 762-767. https://doi.org/10.2337/diabetes.45.6.762
  8. Ishibashi, k., N. N. Miura and Y. Adachi, 2001. Relationship between solubility of grifolan, fungal 1, 3-beta- D-glucan, and productiom of tumor necrosis factor by macrophage in vitro. Biosci. biotechnol. Biochem. 65(9), 1993-2000. https://doi.org/10.1271/bbb.65.1993
  9. Kang, M. H., J. H. Lee, J. H. Kim and H. K. Chung. 2004. Effects of acorn supplementation on lipid profiles and antioxidant enzyme activities in high fat diet-induced obese rats. The Kor. Nutr. Soc. 37(3), 169-175.
  10. Kovachich, G. B. and O. P. Mishra. The effet of ascorbic acid in malondialdehyde formation, $K^+$, $Na^+$ and water content of brain slices. Exp. Brain. Res. 50, 62-68.
  11. Kubo, K., H. Aoki and H. Nanba. 1994. Anti- diabetic activity present in the fruit body of Grifola frondosa (Maitake). I. Bio. Pharm. Bull. Aug. 17(8), 1106-1110. https://doi.org/10.1248/bpb.17.1106
  12. Lee, H. J. and J. B. Koh. 2003. Effect of liquid culture of Agaricus blazei murill on lipid metabolism and enzyme activities in rats fed high fat diet. The Kor. Nutr. Soc. 36(4), 352-358.
  13. Lee. M. Y., M. K. Kim, J. G. Shin and S. D. Kim. 2004. Dietary effect of hemicellulose from soy fiber on blood glucose and cholesterol content in streptozotocin-induced diabetic rats. J. Kor. Soc. Food. Sci. Nutr. 33(7), 1119-1125. https://doi.org/10.3746/jkfn.2004.33.7.1119
  14. Lee, S. C., D. R. Ryu, H. J. Kim, T. H. Yoo, H. Y. Choi, J. S. Kim, T. I. Chang, J. E. Lee, J. T. Park, Y. W. Moon, B. C. Kim, K. H. Choi, H. U. Lee, D. S. Han and S. W. Kan. 2004. Clinical characteristics of non-diabetic renal disease in type 2 diabetic patients. The Kor. J. Nephrol. 23(6), 949-954.
  15. Lee, S. Y. and T. S. Kang. 1999. Structural analysis of the antitumor active exo-polysaccharide produced by submerged cultivation of Ganoderma lucidum mycelium. The Kor. J. Mycol. 27(1), 76-81.
  16. Oh, J. H., H. J. Yoo, S. Y. Park, O. H. Ryu, S. S. Park, S. B. Kwon, H. U. ki, K. W. Lee, N. H. Kim, K. M. Choi, D. R. Cha, S. H. Baik and D. S. Choi. 2004. Plasma and urinary vascular endothelial growth factor and diabetic nephropathy in type 3 diabetes millitus. J. Kor. Diabetes Assoc. 28, 111-121.
  17. Ohno, N., Y. Egawa and T. Hashimoto. 1996. Effect of bata- glucans on the nitric oxide synthesis by peritoneal macrophage in mice. Biol. Pharm. Bull. Apr. 19(4), 608-612. https://doi.org/10.1248/bpb.19.608
  18. Okazaki, M., Y. Adachi and N. Ohno. 1995. Structure-activity relationship of (1$\rightarrow$3)-beta-Dglucans in the in the induction of cytokine production from macrophages, in vitro. Biol. Pharm. Bull. Oct. 18(10), 1320-1327. https://doi.org/10.1248/bpb.18.1320
  19. Satho, K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin. Chim. Acta. 90(1), 37-43. https://doi.org/10.1016/0009-8981(78)90081-5
  20. Smith, O. L. K., C. Y. Wong and R. A. Gelfand. 1989. Skeletal muscle proteolysis in rat with acute streptozotocin- induced diabetes. Diabetes 38, 1117-1122. https://doi.org/10.2337/diabetes.38.9.1117
  21. Talpur, N., B. Echard and A. Dadgor. 2002. Errect of Maitake mushroom fraction on blood pressure of zucker fatty acid. Res. Commun. Mol. Pathol. Pharmacol. 112, 68-82.
  22. Urano, S., H. Midori, N. Tochihi, M. Matsuo, M. Shiraki and H. Ito. 1991. Vitamin E and the susceptibility of erythrocytes and reconstituted liposome to oxidative stress in aged diabetics. Lipids 26, 58-62. https://doi.org/10.1007/BF02544025
  23. Wolff, S. P. 1987. The potential role of oxidative stress in diabetes and its complications: novel implications for theory and therapy. pp. 167-220, In: Crabbe MJC, ed. Diabetic complications: scientific and clinical aspects. New York. Churchill. Livingstone.

피인용 문헌

  1. Physiological activitive of Grifola frondosa by log cultivation and bottle cultivation vol.13, pp.3, 2015, https://doi.org/10.14480/JM.2015.13.3.185