Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Changes of Glycosylation Pattern in Aging Rat Kidneys as Revealed with Lectin Conjugates

성장과정 중 흰쥐 신장의 복합당질 변화에 대한 연구

  • Gil, Young-Gi (Department of Anatomy, Medical School, Kosin University) ;
  • Kim, Keun-Ha (Department of Anatomy, Medical School, Kosin University) ;
  • Choi, Byung-Tae (Department of Anatomy, School of Oriental Medicine, Pusan National University)
  • 길영기 (고신대학교 의과대학 해부학교실) ;
  • 김근하 (고신대학교 의과대학 해부학교실) ;
  • 최병태 (부산대학교 한의학전문대학원 해부학교실)
  • Published : 2007.10.30
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Abstract

The changes of glycoconjuagates (GCs) in rat kidney due to maturation were studied from samples of fetal and postnatal kidneys by lectin histochemistry. Rat kidneys of perinatal ages and adults were fixed in 4% paraformaldehyde and were stained with nine kinds of biotinylated lectins. The immature forms of the renal developmental stage such as vesicles and ureteric bud were observed in the cortex as late as day 14 of postnatal life, but the histological appearance of the weaning kidney was similar to that observed in adults. As for histochemical properties of GCs in the glomeruli, Con A affinity tended to increase with aging, but both RCA-1 and LCA affinities showed a transient increase in immature glomeruli of neonatal rats. DBA affinity with SBA, PNA, BSL-1 and RCA-1, additional Con A one in proximal tubule, were increased in both proximal and distal tubules according to maturation. In contrast to this, transient intensive LCA affinity were demonstrated in immature proximal and distal tubule of neonatal rats. In the collecting tubules, DBA, SBA, PNA and sWGA affinities tended to increase according to maturation, but transient increase for BSL-1, RCA-1 and LCA affinities were detected in neonatal rats. The present results suggest that the mature glycosylation pattern of the kidney undergoes profound changes during maturation and is probably associated with functional maturation of the kidney.

성장과정 중 흰쥐 신장에서 나타나는 복합당질의 변화를 알아보기 위해 18일 태자부터 성체에 이르는 신장을 형태적 관찰과 더불어 9가지 lectin (SBA, DBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, LCA 및 Con A)으로 검색하였다. 신장 발생단계에서 성숙한 신원구조와 함께 미성숙한 구조물 즉 소포와 요관아 등이 생후 14일에 이르기까지 관찰되었으며 생후 21일에 이르러 성체와 유사한 구조적 특성을 보였다. 복합당질의 변화를 보면 사구체에서 RCA-1, LCA및 Con A에 반응을 나타내며 RCA-1 및 LCA는 태자와 신생쥐에서 일시적으로 증가하다 성체에서 관찰되지 않으나 Con A는 성장과 더불어 증가하였다. 근위곡요세관은 UEA-1을 제외한 모든 lectin에 반응하며 DBA, SBA, PNA, BSL-1, RCA-1 및 Con A반응이 성장과 더불어 증가하며 특히 RCA-1과 BSL-1반응이 현저하였다. 이에 비해 sWGA와 LCA반응은 성장과정에 일시적으로 증가하며 성체에 이를수록 감소하였다. 원위곡요세관도 근위곡요세관 유사하게 DBA, SBA, PNA, BSL-1 및 RCA-1반응은 성숙과 함께 증가하나 LCA반응은 성숙과정에 일시적으로 증가하며 성체에서 감소하였다. 집합관에서는 DBA, SBA, PNA, sWGA반응이 성숙과 동시에 증가하나 BSL-1, RCA-1, LCA반응은 미성숙관에서 일시적으로 증가하였다. 이상의 반응으로 보아 신장발생과정에서 형태적 기능적 성숙과 함께 다양한 복합당질의 변화를 보이는데 대체로 성숙에 따라 반응이 증가하는 복합당질군과 미성숙기에 일시적으로 증가하며 성체에서 감소하는 복합당질군으로 대별할 수 있었다. 이러한 출생전후 복합당질의 변화는 신장의 기능적 성숙과정과 연관성을 가지며 발생과정에서 현저한 변화를 나타내는 복합당질은 정상 신장발생에 대한 표지인자로 유용할 것이다.

Keywords

References

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