DOI QR코드

DOI QR Code

히아신스(cv. Pink Pearl)의 인편 기내 배양시 효과적인 자구의 재생과 생장

Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro

  • 이경순 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 정정한 (동아대학교 생명자원과학대학 생명공학과) ;
  • 정용모 (경상남도 농업기술원 하훼육종연구소) ;
  • 김도훈 (동아대학교 생명자원과학대학 유전공학과) ;
  • 정순재 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 남재성 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 김경태 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 이영병 (동아대학교 생명자원과학대학 식물생명공학과)
  • 발행 : 2007.10.30

초록

히아신스 Pink Pearl 품종의 인편배양에서 자구의 재생과 생장은 1.0 mg/l와 3.0 mg/l의 농도에서 IAA보다 IBA가 더 효과적이었으며, 절편체의 기부가 배지에 삽식되는 정상적인 치상 방향이 역방향의 치상 방법에 비해 자구의 재생과 뿌리의 생장에 더 효율적인 것으로 나타났다. 계대배양에서 재생된 자구와 뿌리의 생장은 한천배지보다 펄라이트가 첨가된 액체배지에서 증가되었다. 이와 같은 히아신스 Pink Pearl 품종의 기내배양의 경우 자구 재생을 위한 초대배양은 한천 고체배지를, 재생 된 자구의 생장은 펄라이트의 액체배지를 사용하는 2 단계의 교대배양 시스템이 더 유용함을 시사한다.

The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l). The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.

키워드

참고문헌

  1. Bach, A. 1992. Micropropagation of hyacinths (Hyacinthus orentalis L.), pp. 144-159, In Bajaj, Y.P.S. (ed.), Biotechnology in Agriculture and Forestry, Vol. 20, Springer-Verlag, Berlin - Heidelberg - New York.
  2. Chung, C. H., Y. M. Chung, S. J. Yang, E. K. Ko, S. J. Jeoung, J. S. Nam, G. T. Kim and Y. B. Yi. 2006. Effects of storage temperature and sucrose on bulblet growth, starch and protein contents in in vitro cultures of Hyacinthus orientalis. Biol. Plant 50, 346-351. https://doi.org/10.1007/s10535-006-0048-7
  3. Chung, J. D., C. K. Chun, E. M. Lee and K. Y. Paek. 1981. In vitro propagation of Hyacinthus orientalis L. II. Effect of auxin on callus formation and bulblet differentiation from bulb scale segments. J. Kor. Plant Tissue Cult. 8, 45-48.
  4. Hackett, W. P. 1969. Aseptic multiplications of lily bulblets from bulb scales. Proc. Int. Plant Prop. Soc. 19, 105-108.
  5. Heller, R. 1953. Recherche sur la nutrition minerale des tissus vegetaux cultivés 'in vitro.' Ann. Sci. Nat. Biol. Veg. 14, 1-123.
  6. Hussey, G. 1975a. Propagation of hyacinths by tissue culture. Sci. Hort. 3, 21-28. https://doi.org/10.1016/0304-4238(75)90032-1
  7. Hussey, G. 1975b. Totipotency in tissue explants and callus of some members of Liliaceae, Iridaceae, and Amarylliaceae. J. Expt. Botan. 26, 253-262. https://doi.org/10.1093/jxb/26.2.253
  8. Hutchinson, J. F. 1984. Factors affecting shoot proliferation and root initiation in organ cultures of the apple 'Northern Spy'. Sci. Hort. 22, 347-358. https://doi.org/10.1016/S0304-4238(84)80006-0
  9. Kim, K. W. and A. De Hertogh. 1997. Tissue culture of ornamental flowering bulbs (Geophytes), pp. 87-169, In Jules Janick (ed.), Horticultural Reviews, Vol. 18, Wiley, New York.
  10. Lian, M. L., D. Chakrabarty and K. Y. Paek. 2003. Bulblet formation from bulbscale segments of Lilium using bioreactor system. Biol. Plant 46, 199-203. https://doi.org/10.1023/A:1022890208500
  11. Mirici, S., İ. Parmaksiz, S. Ozcan, C. Sancak, S. Uranbey, E. Sarihan, A. Gumuscu, B. Gurbuz, N. Arslan. 2005. Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana. Plant Cell, Tissue and Organ Cult. 80, 239-246. https://doi.org/10.1007/s11240-004-3016-y
  12. Paek, K. Y. and T. A. Thorpe. 1990. Hyacinth, pp. 479-508, In Ammirato, P. V., D. A. Evans, W. R. Sharp and Y. P. S. Bajaj (eds.), Handbook of Plant Cell Culture, Vol. 5, McGraw-Hill Publishers, New York.
  13. Pierik, R. L. M. and A. J. M. Post. 1975. Rapid vegetative propagation of Hyacinthus orientalis L. in vitro. Sci. Hort. 3, 293-297. https://doi.org/10.1016/0304-4238(75)90013-8
  14. Pierik, R. L. M. and M. A. Ruibing. 1973. Regeneration of bulblets on bulb scale segments of hyacinth in vitro. Neth. J. Agric. Sci. 21, 129-138.
  15. Pierik, R. L. M. and J. Woets. 1971. Regeneration of isolated bulb scale segments of hyacinth. Acta Hort. 23, 423-428.
  16. Rugini, E. and D. C. Verina. 1983. Micropropagation and difficult to propagate Almond (Prunus amygdalus Batch) cultivar. Plant Sci. Lett. 28, 273-281. https://doi.org/10.1016/S0304-4211(83)80019-4
  17. Vishnevetsky, J., M. Ziv and E. Zamski. 2003. Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro. Plant Cell Rep. 21, 645-650.
  18. Whitehead, H. C. M. and K. L. Giles. 1976. Rapid propagation of poplars by tissue culture methods. Comb. Proc. Int. Plant Prop. Soc. 26, 340-343.
  19. Yi, Y. B., J. K. Park and K. S. Lee. 1996. Effect of medium on regeneration and growth of bulblets from the culture of bulb scale segments of Hyacinthus orientalis L. in vitro. J. Kor. Soc. Hort. Sci. 37, 146-151.
  20. Yi, Y. B., K. S. Lee and C. H. Chung. 2002. Protein variation and efficient in vitro culture of scale segments from Hyacinthus orientalis L. cv. Carnegie. Sci. Hort. 92, 367-374. https://doi.org/10.1016/S0304-4238(01)00297-7