Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro

히아신스(cv. Pink Pearl)의 인편 기내 배양시 효과적인 자구의 재생과 생장

  • 이경순 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 정정한 (동아대학교 생명자원과학대학 생명공학과) ;
  • 정용모 (경상남도 농업기술원 하훼육종연구소) ;
  • 김도훈 (동아대학교 생명자원과학대학 유전공학과) ;
  • 정순재 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 남재성 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 김경태 (동아대학교 생명자원과학대학 식물생명공학과) ;
  • 이영병 (동아대학교 생명자원과학대학 식물생명공학과)
  • Published : 2007.10.30
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Abstract

The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l). The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.

히아신스 Pink Pearl 품종의 인편배양에서 자구의 재생과 생장은 1.0 mg/l와 3.0 mg/l의 농도에서 IAA보다 IBA가 더 효과적이었으며, 절편체의 기부가 배지에 삽식되는 정상적인 치상 방향이 역방향의 치상 방법에 비해 자구의 재생과 뿌리의 생장에 더 효율적인 것으로 나타났다. 계대배양에서 재생된 자구와 뿌리의 생장은 한천배지보다 펄라이트가 첨가된 액체배지에서 증가되었다. 이와 같은 히아신스 Pink Pearl 품종의 기내배양의 경우 자구 재생을 위한 초대배양은 한천 고체배지를, 재생 된 자구의 생장은 펄라이트의 액체배지를 사용하는 2 단계의 교대배양 시스템이 더 유용함을 시사한다.

Keywords

References

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