Journal of Plant Biotechnology

  • 제34권3호
  • /
  • Pages.197-200
  • /
  • 2007
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of Artemisia annua L.

  • Choi, Pil-Son (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Min, Sung-Ran (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ko, Suk-Min (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 발행 : 2007.09.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

키워드

참고문헌

  1. Adkins SW, Shiraishi T, McComb JA Ratanopol S, Kupkanchanakul T, Armstrong LJ, Schults AL (1990) Somaclonal variation in rice- lbmerged tolerance and other agronomic characters. Physiol Plant 80: 647-654 https://doi.org/10.1111/j.1399-3054.1990.tb05692.x
  2. Evans DA (1989) Somaclonal variation- genetic basis and breeding applications. Trend Genet 5: 46-50 https://doi.org/10.1016/0168-9525(89)90021-8
  3. Ferreira JFS, Janick J (1995) Floral morphology of Artemisia annua with special reference to trichomes. Int J Plant Sci 156: 807-815 https://doi.org/10.1086/297304
  4. Ferreira JFS, Janick J (1996) Distribution of artemisinin in Artemisia annua. p. 579-584. In: J Janick (ed) Progress in new crops. ASHS Press, Arlington, VA
  5. Fulzele DP, Sipahimalani AT, Heble MR (1991) Tissue cultures of Artemisia annua: organogenesis and artemisinin production. Phytother Res 5: 149-153 https://doi.org/10.1002/ptr.2650050402
  6. Larkin PJ, Scowcroft WR (1981) Somaclonal variation: A novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197-214 https://doi.org/10.1007/BF02342540
  7. Luo XD, Shen CC (1987) The chemistry, pharmacology and clinical applications of qinghaosu (artemisinin) and its derivatives. Med Res Rev 7: 29-52 https://doi.org/10.1002/med.2610070103
  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. Secor GA, Shepard JF (1981) Variability of protoplast-derived potato clones. Crop Sci. 21: 102-105 https://doi.org/10.2135/cropsci1981.0011183X002100010028x
  10. Vergauwe A, Van Geldre E, Inze D, Van Montagu M, Van den Eeckhout E (1998) Factors influencing Agrobaderium tumefadens-mediated transformation of Artemisia annua L. Plant Cell Rep 18: 105-110 https://doi.org/10.1007/s002990050540

피인용 문헌

  1. The influence of endophytic Penicillium oxalicum B4 on growth and artemisinin biosynthesis of in vitro propagated plantlets of Artemisia annua L. vol.80, pp.1, 2016, https://doi.org/10.1007/s10725-016-0162-2