Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative Evaluation of Radix Astragali through the Simultaneous Determination of Bioactive Isoflavonoids and Saponins by HPLC/UV and LC-ESI-MS/MS

  • Kim, Jin-Hee (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University) ;
  • Park, So-Young (Environmental Toxico-Genomic & Proteomic Center, College of Medicine, Korea University) ;
  • Lim, Hyun-Kyun (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University) ;
  • Park, Ah-Yeon (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University) ;
  • Kim, Ju-Sun (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Youm, Jeong-Rok (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University) ;
  • Han, Sang-Beom (Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University)
  • Published : 2007.07.20
Download PDF
⟨ Previous Next ⟩

Abstract

The three major active isoflavonoids (calycosin-7-O-β -glucoside, isomucronulatol 7-O-β-glucoside, formononetin) and two main saponins (astragaloside I, astragaloside IV) in an extract of Radix Astragali were determined using rapid, sensitive, reliable HPLC/UV and LC-ESI-MS/MS methods. The separation conditions employed for HPLC/UV were optimized using a phenyl-hexyl column (4.6 × 150 mm, 5 μm) with the gradient elution of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm. The specificity of the peaks was determined using a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization (ESI) source that was operated in multiple reaction monitoring (MRM) in the positive mode. These methods were fully validated with respect to the linearity, accuracy, precision, recovery and robustness. The HPLC/UV method was applied successfully to the quantification of three major isoflavonoids in the extract of Radix Astragali. The results indicate that the established HPLC/UV and LC-ESI-MS/MS methods are suitable for the quantitative analysis and quality control of multi-components in Radix Astragali.

Keywords

References

  1. Bensky, D.; Gamble, A. Chinese Herbal Medicine: Maleria Medica, Revised Ed.; Eastland Press: Seattle, 1993
  2. Kajimura, K.; Takagi, Y.; Ueba, N.; Yamasaki, K.; Sakagami, Y.; Yokoyama, H.; Yoneda, K. Biol. Pharm. Bull. 1996, 19, 855 https://doi.org/10.1248/bpb.19.855
  3. Dong, T. T.; Ma, X. Q.; Clarke, C.; Song, Z. H.; Ji, Z. N.; Lo, C. K.; Tsim, K. W. J. Agric. Food Chem. 2003, 51, 6709 https://doi.org/10.1021/jf034278x
  4. Wang, X. J.; Feng, P. Acta Pharmacol. Sin. 2000, 21, 1141
  5. Lee, Y. S.; Han, O. K.; Park, C. W.; Suh, S. I.; Shin, S. W.; Yang, C. H.; Jeon, T. W.; Lee, E. S.; Kim, K. J.; Kim, S. H.; Yoo, W. K.; Kim, H. J. J. Ethnopharmacol. 2003, 84, 193 https://doi.org/10.1016/S0378-8741(02)00298-2
  6. Mills, S.; Bone, K. Principles and Practice of Phytotherapy; Edinburgh: Churchill Livingstone, Scotland, 2000; p 273
  7. Yoshida, Y.; Wang, M. Q.; Liu, J. N. Int. J. Immunopharmacol. 1997, 19, 359 https://doi.org/10.1016/S0192-0561(97)00076-3
  8. Ma, X. Q.; Shi, Q.; Duan, J. A. J. Agric. Food Chem. 2002, 50, 40
  9. Wu, T.; Bligh, S. W. A.; Gu, L.-h.; Wang, Z.-t.; Liu, H.-p.; Cheng, X.-m.; Brandford-White, C. J.; Hu, Z.-b. Fitoterapia 2005, 76, 157 https://doi.org/10.1016/j.fitote.2004.11.006
  10. Zheng, Z. R.; Song, C. Q.; Liu, T.; Hu, Z. B. Acta Pharm. Sin. 1998, 148, 33
  11. Xiao, H. B.; Krucker, M.; Albert, K.; Liang, X. M. J. Chromatogr. A 2004, 1032, 117 https://doi.org/10.1016/j.chroma.2003.09.032
  12. Xiao, H. B.; Krucker, M.; Putzbach, K.; Albert, K. J. Chromatogr. A 2005, 1067, 135 https://doi.org/10.1016/j.chroma.2005.01.015
  13. Ministry of Public Health of the People's Republic of China: Pharmacopeia of the People's Republic of China; 2005; Vol. 1, p 212
  14. Pan, F.; Feng, Y. X.; Chen, S. B.; Gao, G. Y. China J. Chin. Materia Med. 1995, 391, 20
  15. Ganzer, M.; Bedir, E.; Calis, I.; Khan, I. A. Chromatographia 2001, 53, 131 https://doi.org/10.1007/BF02491559
  16. Li, W. K.; Fitzloff, J. F. J. Chromatogr. Sci. 2001, 39, 459 https://doi.org/10.1093/chromsci/39.11.459
  17. Yao, M. C.; Qi, Y.; Bi, K. S.; Wang, X.; Luo, X.; Che, C. T. J. Chromatogr. Sci. 2005, 38, 325 https://doi.org/10.1007/BF02269775
  18. Gu, Y. C.; Wang, G. J.; Fawcett, J. P. J. Chromatogr. B 2004, 801, 285 https://doi.org/10.1016/j.jchromb.2003.11.033
  19. Qi, L. W.; Yu, Q. T.; Li, P.; Li, S. L.; Wang, Y. X.; Sheng, L. H.; Yi, L. J. Chromatogr. A 2006, 1134, 162 https://doi.org/10.1016/j.chroma.2006.08.085
  20. Liang, M.; Zhang, W.; Hu, J.; Liu, R.; Zhang, C. J. Pharm. Biomed. Anal. 2006, 42, 178 https://doi.org/10.1016/j.jpba.2006.03.031
  21. Han, S. B.; Jang, M. S.; Lee, H. J.; Lee, Y.; Yu, C.; Lee, K. R.; Kim, H. Bull. Korean Chem. Soc. 2005, 26, 729 https://doi.org/10.5012/bkcs.2005.26.5.729
  22. Choi, Y. W.; Nam, D.; Kang, K. H.; Ha, K. W.; Han, I. H.; Chang, B. K.; Yoon, M.; Lee, J. Bull. Korean Chem. Soc. 2006, 27, 291 https://doi.org/10.5012/bkcs.2006.27.2.291

Cited by

  1. (red algae) vol.25, pp.14, 2011, https://doi.org/10.1080/14786419.2011.553952
  2. Rapid and reliable method for analysis of raw and honey-processed astragalus by UPLC/ESI-Q-TOF-MS using HSS T3 columns vol.6, pp.19, 2014, https://doi.org/10.1039/C4AY01098A
  3. Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry vol.14, pp.3, 2015, https://doi.org/10.1007/s11101-015-9400-x
  4. Current literature in mass spectrometry vol.43, pp.4, 2008, https://doi.org/10.1002/jms.1326
  5. Synthesis of a Complete Series of O-Methyl Analogues of Naringenin and Apigenin vol.28, pp.12, 2007, https://doi.org/10.5012/bkcs.2007.28.12.2527
  6. Simultaneous Determination of Biological Marker Compounds in Ostericum koreanum by HPLC Method and Discrimination by Principal Component Analysis vol.29, pp.12, 2007, https://doi.org/10.5012/bkcs.2008.29.12.2465
  7. Quantitative and Pattern Recognition Analyses for the Quality Evaluationof Herba Epimedii by HPLC vol.30, pp.1, 2009, https://doi.org/10.5012/bkcs.2009.30.1.137
  8. 황기의 추출조건 및 유효성분의 HPLC 분석법 평가 vol.21, pp.6, 2007, https://doi.org/10.7783/kjmcs.2013.21.6.486
  9. 기내배양을 통한 황기 부정근의 생산과 유효성분 분석 vol.23, pp.5, 2007, https://doi.org/10.7783/kjmcs.2015.23.5.357
  10. Major bioactive chemical compounds in Astragali Radix samples from different vendors vary greatly vol.16, pp.7, 2007, https://doi.org/10.1371/journal.pone.0254273