대한한의학방제학회지 (Herbal Formula Science)
- 제14권2호
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- Pages.86-96
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- 2006
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- 1229-1218(pISSN)
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- 2288-5641(eISSN)
해당화근(海棠花根) 단기투여(短期投與)가 3T3-L1 전지방세포(前指肪細胞)의 증식(增殖), 분화(分化) 및 성숙(成熟)에 미치는 영향(影響)
A Study on the Short Term Effect of Rossa rugosae Radix on Proliferation, Differentiation & Maturation of 3T3-L1 Preadipocyte
- Park, Jong-Hyo (Dept. of Oriental Medicine Graduate School of Kyungwon University) ;
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Kim, Dong-Woo
(Dept. of Oriental Medicine Graduate School of Kyungwon University)
- 발행 : 2006.12.30
초록
Objectives : This study wad designed to elucidate the short term effect of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 Preadipocyte. Methods : 3T3-L1 preadipocytes obtained from Korean Cell line Bank were cultured in a Dulbecco's modified eagle medium(DMED) culture colution containing 10% fetal bovine serum(FBS) and various concentration of aqueous extract of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 preadipocytes were investigate after treatment for 24 hours b measuring MTT, Oil Red O and latate dehydrogenase activity.. Results : The Rossa rugosae Radix extract inhibited significantly the proliferation of 3T3-L1 preadipocytes and tended to increase latate dehydrogenase activity in the media of differentiated 3T3-L1 preadipocytes & matured 3T3-L1 preadipocytes. the extract also inhibit the lipid accumulation of differentiated and maturaion of 3T3-L1 preadipocytes, suggesting that Rossa rugosae Radix has anti-obesity effect: however further in vivo study is needed to demonstrate its pharmacological effects.