The Korea Journal of Herbology (대한본초학회지)
- Volume 21 Issue 4
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- Pages.27-36
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- 2006
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
Anticancer effect of Rheum Rhizoma on human liver cancer HepG2 cells
간암 세포주 HepG2에 대한 대황 추출물의 항암효과
- Yun, Hyun-Joung (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Hwang, Seong-Goo (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Yun, Hyung-Joong (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Kim, Chang-Hyun (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Seo, Gyo-Soo (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Park, Won-Hwan (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
- Park, Sun-Dong (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University)
- 윤현정 (동국대학교 한의과대학 방제학교실) ;
- 황성구 (동국대학교 한의과대학 방제학교실) ;
- 윤형중 (동국대학교 한의과대학 방제학교실) ;
- 김창현 (동국대학교 한의과대학 방제학교실) ;
- 서교수 (동국대학교 한의과대학 방제학교실) ;
- 박원한 (동국대학교 한의과대학 방제학교실) ;
- 박선동 (동국대학교 한의과대학 방제학교실)
- Published : 2006.12.30
Abstract
Objectives : This study was performed for the investigation of anticancer effects of methanol extract of Rheum Rhizoma (MeOH-RR) on a human liver cancer cell line (HepG2). Methods : To study the cytotoxic effect of MeOH-RR on HepG2 cells, the cell viability was determined by XTT reduction method and trypan blue exclusion assay. The cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, and the activation of procaspase-3, -8 and -9 were examined by western blot analysis. Furthermore, MeOH-RR-induced apoptosis was confirmed by DNA fragmentation. The release of cytochrome c from mitochondria to cytosol, the level of Bcl-2 and Bax were examined by western blot analysis. Results : MeOH-RR reduced proliferation of HepG2 cells in a dose-dependent manner at 24 h and 48 h treatment. MeOH-RR induced the activation of caspase-3, -8, and -9 and the cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3. Furthermore, treatment with MeOH-RR resulted in internucleosomal DNA fragmentation, evidenced by the formation of a DNA ladder on agarose gel, a hallmark of cells undergoing apoptosis. MeOH-RR downregulated Bcl-2, upregulated Bax, and increased the release of cytochrome c from the mitochondria into cytosol in a dose-dependent manner. Moreover, MeOH-RP increased caspase-3 activity. Conclusion : There results suggest that MeOH-RR induce apoptosis via mitochondrial pathway and caspase-3-dependent pathway in HepG2 cells. There results suggest that MeOH-RR is potentially useful as a chemotherapeutic agent in human liver cancer.