Journal of Korean Traditional Oncology (대한암한의학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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Study on the Effects of Jiaweicitaowan (加味慈桃丸) Ingredients on Angiogenic Inhibition

가미자도환(加味慈桃丸) 구성약물(構成藥物)의 혈관신생(血管新生) 억제효과(抑制效果)에 관한 연구(硏究)

  • Wang, Teh-Chung (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
  • 왕덕중 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 강희 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 심범상 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소)
  • Published : 2006.12.29
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Abstract

Jiaweicitaowan (JWCTW) has been used to inhibit recurrence and metastasis of cancer in clinical practice. Further study has shown its anti-metastatic and anti-angiogenic effects. By applying in vitro and in vivo anti-angiogenic evaluation model, the author assayed the each ingredients of JWCTW. The author performed the following experiments and the results are listed as below: Cell viability assay showed that the viability was almost identical between that of the control and that of the ingredients extract 40 ${\mu}g/m{\ell}$ treated, except of hexane fraction of Curcumae Radix (40 ${\mu}g/m{\ell}$, 2.0% of control), ethylacetate fraction (40 ${\mu}g/m{\ell}$, 26.7%), butanol fraction (20 ${\mu}g/m{\ell}$, 87.2%; 40 ${\mu}g/m{\ell}$, 12.5%) of Cremastrae appendiculatae Tuber, water fraction of Persicae Semen (40 ${\mu}g/m{\ell}$, 82.7%), ethylacetate fraction of Hippocampus (40 ${\mu}g/m{\ell}$, 85.3%). The results of gelatin zymogram assay showed that the ingredients of JWCTW decreases the gelatinolytic activity of MMP-9 from ECV304, at the concentration of 10 ${\mu}g/m{\ell}$. In in vitro invasion assay, the ingredients of JWCTW effectively inhibited the invasion of cancer cells as compared with the control (+PMA) groups. In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of 40 ${\mu}g/m{\ell}$. In ex vivo rat aortic ring assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of 40 ${\mu}g/m{\ell}$. According to the above research, the anti-angiogenic effects of the ingredients of JWCTW was proved and it suggested that the more effective prescription for anti angiogenesis could be developed.

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