Journal of Microbiology and Biotechnology

  • 제16권9호
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  • Pages.1468-1471
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Sequence-Based Screening for a Putative ${\gamma}$-Butyrobetaine Hydroxylase Gene from Neurospora crassa

  • Hur Min-Sang (Division of Animal Science and Biotechnology, Sangji University) ;
  • Cho Jae-Yong (Division of Animal Science and Biotechnology, Sangji University)
  • 발행 : 2006.09.01
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초록

The last step in L-carnitine biosynthesis in eukaryotic organisms is mediated by ${\gamma}$-butyrobetaine hydroxylase (EC1.14.11.1), a dioxygenase that converts ${\gamma}$-butyrobetaine to L-carnitine. This enzyme was previously identified from rat liver and humans, and the peptide sequence of human ${\gamma}$-butyrobetaine hydroxylase was used to search the Neurospora crassa genome database, which led to an identification of an open reading frame (ORF) consisting of 1,407 bp encoding a polypeptide of 468 amino acids. When this protein was expressed in Saccharomyces cerevisiae, the crude cell-free extract exhibited ${\gamma}$-butyrobetaine hydroxylase activity.

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