Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of N-Acetylmuramoyl-L-Alanine Amidase Gene (amiB) from Vibrio anguillarum and the Effect of amiB Gene Deletion on Stress Responses

  • Ahn Sun-Hee (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim Dong-Gyun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Jeong Seung-Ha (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Hong Gyeong-Eun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kong In-Soo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2006.09.01
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Abstract

We identified a gene encoding the N-acetylmuramoyl L-alanine amidase (amiB) of Vibrio anguillarum, which catalyzes the degradation of peptidoglycan in bacteria. The entire open reading frame (ORF) of the amiB gene was composed of 1,722 nucleotides and 573 amino acids. The deduced amino acid sequence of AmiB showed a modular structure with two main domains; an N-terminal region exhibiting an Ami domain and three highly conserved, continuously repeating LysM domains in the C-terminal portion. An amiB mutant was constructed by homologous recombination to study the biochemical function of the AmiB protein in V. anguillarum. Transmission electron microscopy (TEM) revealed morphological differences, and that the mutant strain formed trimeric and tetrameric unseparated cells, suggesting that this enzyme is involved in the separation of daughter cells after cell division. Furthermore, inactivation of the amiB gene resulted in a marked increase of sensitivity to oxidative stress and organic acids.

Keywords

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