Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Alkalophilic Bacillus sp. S-1013 Producing Non-Cariogenicity Sugar Fuc($1{\to}4$)gaINAc($2{\to}6$)NeuAc and Optimization of Culture Condition for Its Production

비우식성 당 Fuc($1{\to}4$)gaINAc($2{\to}6$)NeuAc를 생산하는 호알칼리성 Bacillus sp. S-1013의 동정 및 생산조건의 최적화

  • Ryu Il-Hwan (College of Life Science and Natural Resources, Wonkwang University) ;
  • Kim Sun-Sook (Department of Dental Hygienic, Suwon Wonen's College) ;
  • Lee Kap-Sang (College of Life Science and natural Resources, Wonkwang University) ;
  • Lee Eun-Sook (Department of Dental Hygienic, Gimcheon College)
  • 류일환 (원광대학교 생명자원과학대학 식품환경학부) ;
  • 김선숙 (수원여자대학 치위생과) ;
  • 이갑상 (원광대학교 생명자원과학대학 식품환경학부) ;
  • 이은숙 (김천대학 치위생과)
  • Published : 2006.09.01
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Abstract

The study was performed to identification of producing microbe Non-Cariogenicity Sugar (NCS; Fuc($1{\to}4$) gaINAc($2{\to}6$)NeuAc) with anti-caries activity, and to optimization of production condition. A typical strain which produced the NCS was identified alkalophilic Bacillus sp. S-1013 through the results of morphological, biochemical and chemotaxonomic characteristics and 16S rDNA sequencing. The optimal medium composition for the maximal production of the NCS from alkalophilic Bacillus sp. S-1013 was as follow: soluble starch 30 g, dextrin 15 g, yeast extract 5 g, peptone 10 g, $K_{2}HPO_4$ 2 g in a liter of distilled water. Optimal temperature and pH were 25 and 11.0, respectively. The highest production of NCS was shown 60 hrs cultivation using the optimal medium, and then NCS productivity and dry cell weight of culture broth increased 4.24 and 2.67 time than initial medium, respectively.

비우식성 당 NCS(Fuc($1{\to}4$)galNAc($2{\to}6$)NeuAc)를 생산하는 미생물을 동정하고 이 미생물이 생산하는 비우식성 당의 생산조건을 최적화하기 위하여 본 연구를 수행하였다. NCS를 생산하는 균주는 형태학적, 생화학적, 화학분류학적 특성 및 16S-rRNA 염기서열 결정을 통하여 Bacillus sp. S-1013로 동정되었다. 또한, 이 균주에 의한 NCS의 최적 배지조성은 soluble starch 3.0%, dextrin 1.5%, yeast extract 0.5%, pepton 1.0%, $K_{2}HPO_4$ 0.2%였으며, 최적온도 및 pH는 각각 25와 11.0이였다. 최적배지에서 NCS 및 균체의 생산은 기본배지에 비해 각각 4.24배 및 2.67배 증가하였다.

Keywords

References

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