Biomedical Science Letters (대한의생명과학회지)
- Volume 12 Issue 3
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- Pages.139-143
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- 2006
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Development of a Unidirectional Expression Vector: in a Search of Suppressor against a Cell Death-Inducing Protein, Jpk
- Kong Kyoung-Ah (Department of Anatomy, Embryology Lab., Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
- Park Sung-Do (Division of Zoonoses, Center for Immunology and Pathology, National Institute of Health, Korea Center for Disease Control and Prevention) ;
- Kim Myoung-Hee (Department of Anatomy, Embryology Lab., Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine)
- Published : 2006.09.01
Abstract
Jopock (Jpk) has previously been ascertained that induces both bacterial and mammalian cell death. The Escherichia coli cells expressing Glutathion S-transferase (GST) fused Jpk showed elongated phenotype and inhibited cell growth which led eventual cell death. In an attempt to search the genetic suppressor of the lethal protein Jpk in bacterial cells, we constructed a unidirectional protein expression vector inserting tac promoter next to the C-terminus Jpk in pGEX-Jpk. The function of additional tac promoter was confirmed by substituting lac promoter in Plac-TOPO plasmid. The cells harboring plac- TOPO, which regulates