전기화학회지 (Journal of the Korean Electrochemical Society)

  • 제9권3호
  • /
  • Pages.113-117
  • /
  • 2006
  • /
  • 1229-1935(pISSN)
  • /
  • 2288-9000(eISSN)
한국전기화학회 (The Korean Electrochemical Society)
권호 표지
DOI QR코드

DOI QR Code

Electrochemical Behaviors of ABTS2- on the Thiol-modified Gold Electrodes

  • 발행 : 2006.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The electrochemical properties of the redox mediator, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) ($ABTS^{2-}$) were studied using cyclic voltammetry. The measured potentials (${E^o}'$ vs SCE) of the two redox couples of ABTS are 0.45 V for $ABTS^{2-}/ABTS^{\cdot-}$ and 0.87 V for $ABTS^{\cdot-}/ABTS^0$. The rate constant for heterogeneous electron transfer and the diffusion coefficients for $ABTS^{2-}$ are $5x10^{-3}cm\;s^{-1}$ and $3.1x10^{-6}cm^2\;s^{-1}$, respectively. Our interest in $ABTS^{2-}$ stems from the fact that this molecule functions as a substrate to the copper oxidase, laccase, by providing the reducing equivalents necessary for the biocatalyzed reduction of dioxygen to water. Consequently, when laccase is tethered to an electrode surface or dissolved in solution, $ABTS^{2-}$ can be used to quantify enzyme activity electrochemically.

키워드

참고문헌

  1. F. Xu, J. Biol. Chem., 272, 921 (1997)
  2. A. Naqui and S. D. Varfolomeev, FEBS Lett., 113, 157 (1980) https://doi.org/10.1016/0014-5793(80)80581-3
  3. G. B. Koudelka and M. J. Ettinger, J. Biol. Chem. 263, 3698 (1988)
  4. A. Dalmia, R. F. Savinell, and C. C. Liu, J. Electro. Chem. Soc., 143, 1827 (1996) https://doi.org/10.1149/1.1836911
  5. D. Leech and F. Daigle, Analyst, 123, 1971 (1998) https://doi.org/10.1039/a803313g
  6. F. Trudeau, F. Daigle, and D. Leech, Anal. Chem., 69, 882 (1997) https://doi.org/10.1021/ac960554d
  7. R. R. Malkin and B. G. Malmstrom, Adv. Enzymol., 33, 177 (1970)
  8. L.-E. Andreisson. R. Branden. and B. Reinhanunar. Biochim. Biophys. Acta, 438 (1976)
  9. M. R. Tarasevich, A. I. Yaropolov, V. A. Bogdanovskaya, and S. D. Varfolomeev, J. Electroanal. Chem., 104, 393 (1979) https://doi.org/10.1016/S0022-0728(79)81047-5
  10. L. Venkatasubramanian and P. Maruthamuthu, Int. J. Chem. Kinetics, 21, 399 (1989) https://doi.org/10.1002/kin.550210604
  11. R. E. Childs and W. G Bardsley, J. Biochem., 145, 93 (1975) https://doi.org/10.1042/bj1450093
  12. P. A. Adams, J. Chem. Soc. Perkin Trans., 2, 1407 (1990)
  13. P. Maruthamuthu, D. K. Sharma, and N. Serpone, J. Phys. Chem., 99, 3636 (1995) https://doi.org/10.1021/j100011a034
  14. R. Bourbonnais and M. G. Paice, Appl. Microbiol. Biotechnol., 36, 823 (1992)
  15. V. A. Bogdanovskaya, E. F. Gavrilova, and M. R. Tarasevich, Elektrokhirniya, 22, 742 (1986)
  16. C.-W. Lee, H. B. Gray, F. C. Anson, and B. G. Malmstrom, J. Electroanal. Chem., 172, 289 (1984) https://doi.org/10.1016/0022-0728(84)80193-X
  17. J. Ohkawa, N. Okada, A. Shinmyo, and M. Takano, Proc. Natl. Acad Sci., 86, 1239 (1989) https://doi.org/10.1073/pnas.86.4.1239
  18. G. T. R. Palmore and H.-H. Kim, J. Electroanal. Chem., 464, 110 (1999) https://doi.org/10.1016/S0022-0728(99)00008-X
  19. E. Steckhan and T. Kuwana, Ber. Bunsen., 78, 253 (1974)
  20. A. J. Bard and L. R. Faulkner, 'Electrochemical Methods', John Wiley & Sons, New York (2000)
  21. R. S. Nicholson and L. Shain, Anal. Chem., 36, 706 (1964) https://doi.org/10.1021/ac60210a007
  22. R. S. Nicholson, Anal. Chem., 37, 1351 (1965) https://doi.org/10.1021/ac60230a016
  23. J. Heinze, Ber. Bunsenges. Phys. Chem., 85, 1096 (1981) https://doi.org/10.1002/bbpc.19810851204
  24. C. R. Cantor and P. R. Schimmel, 'Biophysical Chemistry', Freeman, New York, (1980)

피인용 문헌

  1. A nitrogen dioxide delivery system for biological media vol.56, 2013, https://doi.org/10.1016/j.freeradbiomed.2012.10.534