Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats

Rat의 방사선 조사성 구내염에 대한 Recombinant Human Epidermal Growth Factor (rhEGF)의 효과

  • Jung Kwon-Il (Departments of Toxicology and Pharmacology, Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co., LTD.) ;
  • Kim Sun-Hee (Departments of Protein Drug Development, Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co., LTD.) ;
  • Moon Soo-Young (Departments of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Kim Yeon-Wha (Departmants of Plastic Surgery Oncology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Hong Joon-Pio (Departmants of Plastic Surgery Oncology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Kim Hyun-Sook (Department of Radiology, Gyeongsang National University College of Medicine) ;
  • Lee Sang-Wook (Departments of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center)
  • 정권일 (대웅제약 생명공학연구소 약리독성팀) ;
  • 김선희 (대웅제약 생명공학연구소 단백질의약팀) ;
  • 문수영 (울산대학교 의과대학 서울아산병원 방사선종양학과) ;
  • 김연화 (울산대학교 의과대학 서울아산병원 성형외과) ;
  • 홍준표 (울산대학교 의과대학 서울아산병원 성형외과) ;
  • 김현숙 (경상대학교 의과대학 방사선과학교실) ;
  • 이상욱 (울산대학교 의과대학 서울아산병원 방사선종양학과)
  • Published : 2006.03.01

Abstract

Purpose: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model. Materials and Methods: Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF-treated groups. Rats were topically applied with rhEGF (15 or $30{\mu}g/oral$ cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation. Results: rhEGF-treated groups (15 or $30{\mu}g/oral$) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats. Conclusion: These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.

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