Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii

  • Kim Ji-Young (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Ahn Myoung-Hee (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Song Hyun-Ouk (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Choi Jong-Hak (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Ryu Jae-Sook (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Min Duk-Young (Department of Parasitology, Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Cho Myung-Hwan (Department of Biological Science, College of Science, Konkuk University)
  • Published : 2006.09.01
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Abstract

This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and $MIP-1\alpha$, and enzyme, COX-2/prostaglandin $E_2(PGE_2)$ in infected cells via western blot, $[^3H]-uracil$ incorporation assay, ELISA and RT-PCR. The phosphorylation of ERK1/2 and p38 in infected HeLa cells was detected at 1 hr and/or 6 hr postinfection (PI). Tachyzoite proliferation was reduced by p38 or JNK MAPK inhibitors. MCP-1 secretion was enhanced in infected peritoneal macrophages at 6 hr PI. $MIP-1\alpha$ mRNA was increased in macrophages at 18 hr PI. MCP-1 and $MIP-1\alpha$ were reduced after treatment with inhibitors of ERK1/2 and JNK MAPKs. COX-2 mRNA gradually increased in infected RAW 264.7 cells and the secretion of COX-2 peaked at 6 hr PI. The inhibitor of JNK suppressed COX-2 expression. $PGE_2$ from infected RAW 264.7 cells was increased and synthesis was suppressed by PD98059, SB203580, and SP600125. In this study, the activation of p38, JNK and/or ERK1/2 MAPKs occurred during the invasion and proliferation of T. gondii tachyzoites in HeLa cells. Also, increased secretion and expression of MCP-1, $MIP-1\alpha$, COX-2 and $PGE_2$ were detected in infected macrophages, and appeared to occur via MAPK signaling pathways.

Keywords

References

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