The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Chrysanthemum stunt viroid Induces the Accumulation of Small RNAs Associated with RNA Silencing in Infected Chrysanthemum

  • Chung Bong-Nam (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim Ki-Taek (National Horticultural Research Institute, Rural Development Administration)
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Chrysanthemum stunt viroid (CSVd) induced systemic symptoms on chrysanthemum. We detected small RNAs of approximately 22 nucleotides with sequence specificity to CSVd in chrysanthemum infected with CSVd: an indication of the presence of RNA silencing. Regardless of symptom differences associated with CSVd, the small RNAs distributed similarly in amount. Small RNAs were detected with partial-length or full-length probes, indicating that they are not restricted to specific viroid regions but likely representing most of the viroid molecule.

Keywords

References

  1. Baulcombe, D. C. 1996. RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants. Plant Mol. BioI. 32:79-88 https://doi.org/10.1007/BF00039378
  2. Baulcombe, D. C.1999. Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant BioI. 2: 109-113 https://doi.org/10.1016/S1369-5266(99)80022-3
  3. Baulcombe, D. C. 2004. RNA silencing in plants. Nature 431:356-363 https://doi.org/10.1038/nature02874
  4. Branch, A .D. and Robertson, H. D. 1984. A replication cycle for viroids and other small infectious RNA's. Science 223 :540-555 https://doi.org/10.1126/science.223.4636.540
  5. Bussiere, F., Lehoux, J., Thompson, D. A., Skrzeczkowski, L. J. and Perreault, J. P. 1999. Subcellular localization and rolling circle replication of Peach latent mosaic viroid: Hallmarks of Group A Viroids. J. Virol. 73:6353-6360
  6. Canto, T., Cillo, F. and Palukaitis, P. 2002. Generation of siRNA by T-DNA sequences dose not require active transcription or homology to sequences in the plant. Mol. Plant-Microbe Interact. 15:1137-1146 https://doi.org/10.1094/MPMI.2002.15.11.1137
  7. Chung, B. N., Lim, J. H., Choi, S. Y., Kim, J. S. and Lee, E. J. 2005. Occurrence of Chrysanthemum stunt viroid in Chrysanthemum in Korea. Plant Pathol. J. 21:377-382 https://doi.org/10.5423/PPJ.2005.21.4.377
  8. Dalmay, T., Hamilton, A., Rudd, S., Angell, S. and Baulcombe, D. C. 2000. An RNA dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 101:543-553 https://doi.org/10.1016/S0092-8674(00)80864-8
  9. Dalmay, T., Horsefield, R., Braunstein, T. H. and Baulcombe, D. C. 2001. SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis. EMBO J. 20:2069-2077 https://doi.org/10.1093/emboj/20.8.2069
  10. Diener, T. O. 1993. Viroids: The smallest and simplest agent of infectious disease. How do they make plants sick. Intervirology 35:186-195 https://doi.org/10.1159/000150309
  11. Flores, R., Hernandez, C., Martinez de Alba, A. E. and Di Serio, F. 2005. Viroids and viroid-host interactions. Annu. Rev. Phytopathol. 43:117-139 https://doi.org/10.1146/annurev.phyto.43.040204.140243
  12. Hamilton, A. J. and Baulcombe, D. C. 1999. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286:950-952 https://doi.org/10.1126/science.286.5441.950
  13. Itaya, A., Folimonov, A., Matsuda, Y., Nelson, R. S. and Ding, B. 2001. Potato spindle tuber viroid as inducer of RNA silencing in infected tomato. Mol. Plant-Microbe Interact. 11:1332-1334
  14. Lindbo, J. A., Fitzmaurice, W. P. and della-Cioppa, G. 2001. Virus mediated reprogramming of gene expression in plants. Curr. Opin. Plant Biol. 4:181-185 https://doi.org/10.1016/S1369-5266(00)00158-8
  15. Markarian, N., Li, H. W., Ding, S. W. and Semancik, J. S. 2004. RNA silencing as related to viroid induced symptom expression. Arch Virol. 149:397-406 https://doi.org/10.1007/s00705-003-0215-5
  16. Martinez de Alba, A. E., Flores, R. and Hernandez, C. 2002. Two chloroplastic viroids induce the accumulation of small RNAs associated with posttranscriptional gene silencing. J. Virol. 76:13094-13096 https://doi.org/10.1128/JVI.76.24.13094-13096.2002
  17. Mayo, M. A. 1999. Developments in plant virus taxonomy since the publication of the 6th ICTV Report. Arch Virol. 144:1659-1666 https://doi.org/10.1007/s007050050620
  18. Mette, M. F., Aufsatz, W., van der Winden, J., Matzke, M. A. and Matzke, A. J. M. 2000. Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J. 19:5194-5201 https://doi.org/10.1093/emboj/19.19.5194
  19. Papaefthimiou, I., Hamilton, A. J., Denti, M. A., Baulcombe, D. C., Tsagris, M. and Tabler, M. 2001. Replicating Potato spindIe tuber viroid RNA is accompanied by short RNA fragments that are characteristic of post-transcriptional gene silencing. Nucleic Acids Res. 29:2395-2400 https://doi.org/10.1093/nar/29.11.2395
  20. Ratcliff, F. G., MacFarlane, S. A. and Baulcombe, D. C. Gene silencing without DNA:RNA mediated cross protection between viruses. Plant Cell 11:1207-1215 https://doi.org/10.1105/tpc.11.7.1207
  21. Vance, V. and Vaucheret, H. 2001. RNA silencing in plant-defense and counterdefense. Science 292:2277-2280 https://doi.org/10.1126/science.1061334

Cited by

  1. A Current Overview of Two Viroids That Infect Chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid vol.5, pp.4, 2013, https://doi.org/10.3390/v5041099