Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of D-Glucose ${\alpha}$-1-Phosphate Uridylyltransferase (VldB) and Glucokinase (VIdC) Involved in Validamycin Biosynthesis of Streptomyces hygroscopicus var. limoneus KCCM 11405

  • Seo Myung-Ji (Division of Bio.New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Im Eun-Mi (Division of Bio.New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Singh Deepak (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Rajkarnikar Arishma (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Kwon Hyung-Jin (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Hyun Chang-Gu (Division of Bio.New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI)) ;
  • Suh Joo-Won (Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University) ;
  • Pyun Yu-Ryang (Department of Biotechnology, Yonsei University) ;
  • Kim Soon-Ok (Division of Bio.New Drug Development, Central Research Institute, Chem Tech Research Incorporation (C-TRI))
  • Published : 2006.08.01
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Abstract

Aminocyclitol antibiotic validamycin A, a prime control agent for sheath blight disease of rice plants, is biosynthesized by Streptomyces hygroscopicus var. limoneus. Within the validamycin biosynthetic gene cluster, vldBC forms an operon of vldABC with vidA, the gene encoding 2-epi-5-epi-valiolone synthase. Biochemical studies, employing the recombinant proteins from Escherichia coli, established VldB and VldC as D-glucose $\alpha$-1-phosphate uridylyltransferase and glucokinase, respectively. This finding substantiates that the validamycin biosynthetic gene cluster harbors genes encoding the enzymes for UDP-glucose formation from glucose. Therefore, we propose that validamycin biosynthesis employs its own catalysts to generate UDP-glucose, but not depending on the primary metabolism.

Keywords

References

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