International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Influence of Temperature on Microsporidian Multiplication and Spore Production in Various Tissues of Silkworm (Bombyx mori L.) During Larval Development

  • Mohanan N. Madana (Central Sericultural Research and Training Institute, Central Silk Board) ;
  • Krishnan N. (Department of Physiology, Institute of Entomology, Czech Academy of Sciences) ;
  • Mitra P. (Central Sericultural Research and Training Institute, Central Silk Board) ;
  • Das K.K. (DBKV, Dakshin Dinajpur) ;
  • Saratchandra B. (Central Silk Board, BTM Layout) ;
  • Haldar D.P. (Protozoology Laboratory, Department of Zoology, University of Kalyani)
  • Published : 2006.06.01
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Abstract

Multiplication and spore production of three microsporidia viz., Nosema bombycis, Nosema sp. 1 and Nosema sp. 2 in fifth instar larval tissues of silkworm, Bombyx mori L. in two seasons with distinct temperature regimes were studied. Nosema sp. 2 produced significantly (P < 0.01) higher number of spores in various tissues. Among the tissues, spore production was highest in silk gland, followed by fat body and gut. Spore production was significantly (P < 0.01) higher in season-II (Average temperature $29.4{\pm}1.1^{\circ}C$). Maximum spore production was observed 25 days post inoculation (p. i.) in season-I (Average temperature $18.9{\pm}1.1^{\circ}C$), whereas in season-II, it was 14 days p. i. In season-I, spore production was low up to 21 days p. i., then increased sharply. In season-II, there was a steady increase in spore production. The results indicate that the microsporidian multiplication is tissue specific and extremely sensitive to temperature at which the host is reared. It also reveals that, silk gland, fat body and gut are the most appropriate tissues for microscopic identification of microsporidia in the larval stage.

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References

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