International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Inhibitory Activity of Bumblebee Worker (Bombus terrestris L.) Venoms on Nitric Oxide, TNF-${\alpha}$ and IL-6 Production in Lipopolysaccharide-Activated Macrophages

  • Han Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee Kwang-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yeo Joo-Hong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kweon Hae-Yong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Woo Soon-Ok (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoon Hyung-Joo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Baek Ha-Ju (Gyeongsang Buk-Do Government Public Institute of Health and Environmental) ;
  • Park Kwan-Kyu (Department of Pathology, School of Medicine, Catholic University of Daegu)
  • Published : 2006.06.01
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Abstract

To elucidate the composition of bumblebee (Bomb us terrestris) venom (BBV) and the anti-inflammatory effect of BBV. The major components of BBV by LC chromatography and SDS-PAGE were identified. The production of nitric oxide (NO) and proinflammatory cytokines was examined by lipopolysaccharide (LPS) in a macrophage cell line, RAW 264.7 cells, with BBV. BBV inhibits LPS-induced NO in a dose dependent manner. We also found that BBV inhibits proinflammatory cytokine, tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6 production. These findings mean that BBV can be used in controlling macrophages mediated inflammation related disease. Additional studies on the pharmacological aspects of the individual components of BBV are recommended for future trials.

Keywords

References

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