Journal of Biosystems Engineering

  • 제31권2호
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  • Pages.128-134
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  • 2006
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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리스테리아 식중독균 검출을 위한 광학식 바이오센서 개발

Development of a Fiber-Optic Biosensor for the Detection of Listeria monocytogenes

  • Kim G. (National Institute of Agricultural Engineering, RDA) ;
  • Choi K.H. (National Institute of Agricultural Engineering, RDA)
  • 발행 : 2006.04.01
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초록

Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.

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참고문헌

  1. Anderson, G.P., K.D. King, D.S. Cuttino, J.P. Whelan, F.S. Ligler, J.F. MacKrell, C.S. Bovais, D.K. Indyke, R.J. Foch. 1999. Biological agent detection with the use of an airborne biosensor. Field Analytical Chemistry and Technology 3(4-5): 307-314 https://doi.org/10.1002/(SICI)1520-6521(1999)3:4/5<307::AID-FACT9>3.0.CO;2-M
  2. Bhunia, A.K., T. Geng, A. Lathrop, A. Valadez, M.T. Morgan. 2004. Optical immunosensors for detection of Listeria monocytogenes and Salmonella Enteritidis from food. Proceedings of SPIE 5271: 1-6
  3. CDC. 2002. Listeriosis outbreak investigation, CDC Press Release
  4. DeMarco, D.R., E.W. Saaski, D.A. McCrae, D.V. Lim. 1999. Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor. Journal of Food Protection 62(7): 711-716 https://doi.org/10.4315/0362-028X-62.7.711
  5. Donaldson, K.A., M.F. Kramer, and D.V. Lim. 2004. A rapid detection method for Vaccinia virus, the surrogate for smallpox virus. Biosensors and Bioelectronics 20: 322-327 https://doi.org/10.1016/j.bios.2004.01.029
  6. Marazuela, M.D. and M.C. Moreno-Bondi. 2002. Fiber-optic biosensors-an overview. Anal. Bioanal. Chem. 372: 664-682 https://doi.org/10.1007/s00216-002-1235-9
  7. Tims, T.B. and D.V. Lim. 2003. Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. Journal of Microbiological Methods 55: 141-147 https://doi.org/10.1016/S0167-7012(03)00133-7
  8. Tims, T.B., S.S. Dickey, D.R. Demarco, D.V. Lim. 2001. Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. American Clinical Laboratory. Sep.: 28-29

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