죽력지출환(竹瀝枳朮丸)의 메탄올추출 엑기스가 흰쥐의 전뇌허혈에 미치는 영향

Effects of Methanol Extract of Jukryukjichul-hwan on Global Cerebral Ischemia of Rats

  • 류지철 (동의대학교 부속한방병원 심계내과학 교실) ;
  • 김영균 (동의대학교 부속한방병원 심계내과학 교실) ;
  • 권정남 (동의대학교 부속한방병원 심계내과학 교실)
  • Ryu Ji-Cheal (Department of Circulatory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kim Young-Kyun (Department of Circulatory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kwon Jung-Nam (Department of Circulatory Internal Medicine, College of Oriental Medicine, Dong-Eui University)
  • 발행 : 2006.06.30

초록

Objectives : Ischemic brain injury is a worldwide problem that often causes irreversible brain damage. Moreover, prevention of ischemic brain injury is more important than anything else, since after-effects of stroke significantly threat the quality of life. Jukryukjichul-hwan (JRH) is an oriental medicinal formula for stroke patients in Korea. This study evaluated neuroprotective effects of methanol extract of JRH on global cerebral ischemia in rats. Changes of the pyramidal neurons, Bax and TUNEL immuno-positive neurons in CA1 hippocampus were observed using immunohistochemistry. Methods : Sprague-Dawley Rats were induced with temporal global cerebral ischemia (TGI) by occluding the bilateral common carotid artery with hypotension, The rats were divided into 3 groups. We treated one group with methanol extract of JRH after operation, another group before and after the operation. We observed Bax expressions inducing apoptosis of neurons and TUNEL-positive Pyramidal Neurons as an index of survival and apoptosis of pyramidal neurons in CA1 Hippocampus. Results : JRH treatment before and after TGI inhibited Bax expression in CA1 hippocampus. JRH treatment before and after TGI reduced the cell death of pyramidal neurons in CA1 hippocampus. JRH treatment after TGI reduced the cell death of pyramidal neurons in CA1 hippocampus. JRH treatment before and after TGI reduced TUNEL-positive cells in CA1 hippocampus. Conclusion : These results suggest that JRH has a neuroprotective effect (by anti-apoptosis) against cerebral ischemia.

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