International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Expression of DSPP mRNA During Differentiation of Human Dental Pulp-derived Cells (HDPC) and Transplantation of HDPC Using Alginate Scaffold

  • Aikawa, Fumiko (Department of Oral Anatomy, Osaka Dental University) ;
  • Nakatsuka, Michiko (Department of Oral Anatomy, Osaka Dental University) ;
  • Kumabe, Shunji (Department of Oral Anatomy, Osaka Dental University) ;
  • Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kung Hee University) ;
  • Hayashi, Hiroyuki (Department of Endodontics, Osaka Dental University) ;
  • Shin, Je-Won (Department of Oral Anatomy, School of Dentistry, Kung Hee University) ;
  • Iwai, Yasutomo (Department of Oral Anatomy, Osaka Dental University)
  • Published : 2006.09.30
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Abstract

Tissue stem cells are used for the regenerative medicine. In previous study we observed hard tissue formation of human dental pulp-derived cells using alginate scaffold. In this study, we explore the ability to differentiate of the 13th passage cells with glycerol 2-phosphate disodium salt hydrate (${\beta}-GP$) which accelerate calcification. Reverse transcriptase Polymerase Chain Reaction (RT-PCR), transplants using alginate scaffold and histological examination were performed. We observed the expression of DSPP mRNA on day 10 cultured cells with ${\beta}-GP$. In conclusion, the 13th passage cells still have an ability to differentiate into odontoblast-like cells and alginate supports the differentiation of cultured cells in the transplants.

Keywords

References

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