Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Liver Protecting Effect of Pomegranate (Punica granatum) Seed Oil in Mice Treated with $CCl_4$

사염화탄소를 처리한 생쥐에서 석류종자기름의 간보호 효과

  • Kim, Dong-Heui (Department of Basic Science, Wonju College of Medicine, Yonsei University) ;
  • Deung, Young-Kun (Department of Basic Science, Wonju College of Medicine, Yonsei University) ;
  • Lee, Young-Mi (Biotech Research Institute, HDr Co., Ltd.) ;
  • Yoon, Yang-Suk (Biotech Research Institute, HDr Co., Ltd.) ;
  • Kwon, Ki-Rok (Department of Acupuncture, College of Oriental Medicine, Sangji University) ;
  • Park, Dae-Bok (Isoo Research Center, I-Soo Co., Ltd.) ;
  • Park, Seung-Kyu (Department of Parasitology, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Parasitology, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 기초과학교실) ;
  • 등영건 (연세대학교 원주의과대학 기초과학교실) ;
  • 이영미 (HDr 주식회사 바이오연구소) ;
  • 윤양숙 (HDr 주식회사 바이오연구소) ;
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 박대복 (아이수 연구소) ;
  • 박승규 (연세대학교 원주의과대학 기생충학교실) ;
  • 이규재 (연세대학교 원주의과대학 기생충학교실)
  • Published : 2006.09.30
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Abstract

Recently, the pomegranate seed oil (PSO) has been reported to have various efforts including anti-cancer effect. In this study, we examined the liver-protecting effect of the PSO on the hepatotoxicity induced by $CCl_4$ using the BALB/c mice. The PSO was made from dried seeds of black pomegranate (Punica grantum) by heating and squeezing. The expreimental animals were divided into 3 groups; control group treated with olive oil only, experimental group 1 treated with $CCl_4$ only, and experimental group 2 treated with PSO and $CCl_4$. 24 hours after injection of $CCl_4$ into the peritoneal cavity, we collected the blood samples to measure the level of serological factors; aspartate aminotransferase(AST), alanine aminotransferase (ALT), total protein, albumin, total bilirubin, direct bilirubin and alkaline phosphatase. Simultaneously we observed the histological change of liver under the light and electron microscope. As the result, AST and ALT showed $88.7{\pm}14.9IU/L\;and\;22.0{\pm}3.12IU/L$ in the control group, $1963.7{\pm}1212.9IU/L\;and\;4495.4{\pm}2803.6IU/L$ in the experimental group 1, and $432.2{\pm}260.1IU/L\;and\;692.3{\pm}433.1IU/L$ in the experimental group 2. The experimental group 2 showed significant difference as compared with experimental group 1 (P<0.005). In histological study, the experimental group 2 was recovered than experimental group 1 which had abnormal mitochondria, increase of lysosomes, and severe necrosis at the central vein zones. These results indicated that the PSO had the liver protecting effect. However, The further study on the relationship between ingredients of pomegranate seed and liver protecting effect is in need.

최근 석류씨 기름은 항암효과를 포함하여 다양한 효과가 있는 것으로 확인되고 있다. 본 연구는 BALB/c 생쥐에 석류씨 기름을 처리한 후 사염화탄소를 주사하여 석류씨 기름이 간 독성에 어떤 영향을 미치는지 확인하기 위하여 올리브 기름만을 처리한 대조군, 사염화탄소만을 처리한 실험군1, 및 사염화탄소를 처리한 후 석류씨 기름을 구강투여한 실험군2로 나누어 24시간 후 혈액을 채취하여 혈청내 AST (aspartate aminotransferase, SGOT), ALT (alanine aminotransferase, SGPT), total protein, albumin, total bilirubin, direct bilirubin 및 alkaline phosphatase의 함량을 측정하였고 광학현미경과 투과전자현미경으로 조직의 변화를 관찰하였다. AST와 ALT는 대조군에서 각각 $88.70{\pm}14.90$ 및 $22.00{\pm}3.12IU/L$, 실험군 1에서 $1963.70{\pm}1212.90$ 및 $4495.40{\pm}2803.60IU/L$ 그리고 실험군2에서 $432.20{\pm}260.10$ 및 $692.30{\pm}433.10IU/L$이었으며 실험군2에서의 측정값은 실험군 1에 비해 유의한 차이를 보였다(P<0.005). 간조직을 관찰한 결과 사염화탄소를 처리한 실험군1에서는 문맥을 제외한 중심정맥 주위에서 간조직의 심한 응고성 괴사가 관찰되었고, 괴사조직 가장자리 주위로 지방변성을 확인할 수 있었다. 이에 비하여 석류씨 기름을 처리한 군에서 중심정맥 주위의 간실질 조직의 괴사가 실험군1에 비하여 현저히 줄어들었고, 간세포의 지방변성은 계속 관찰되었으나 용해소체의 감소와 미토콘드리아가 실험군1에 비해서 보존되어 있는 것으로 보아 석류씨 기름이 간보호에 효과가 있는 것으로 사료된다.

Keywords

References

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