Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Protective Effects of Activated Charcoal on the Acute Damages of Kidney of Mouse by Lead

급성 납 중독된 생쥐의 신장에서 활성탄의 보호효과

  • Cheong, Min-Ju (Department of Biology, College of Natural Science, Chosun University) ;
  • Roh, Young-Bok (Department of Biology, College of Natural Science, Chosun University)
  • 정민주 (조선대학교 자연과학대학 생물학과) ;
  • 노영복 (조선대학교 자연과학대학 생물학과)
  • Published : 2006.06.30
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Abstract

A protective effect of activated charcoal against the acute lead poisoning of kidney was studied in mice. Mice approximately 30 gm in weight were grouped into the control, lead acetate-treated. and the activated charcoal-treated after lead acetate groups. Lead acetate (60mg/kg) and activated charcoal (40mg/kg) were delivered orally. Serum BUN and creatine were measured and ultrastructural alteration of renal tissues were examined by electron microscopy. Activated charcoal were decreased the increase of serum BUN and Creatinine level induced by lead. Lead acetate-treated renal tissues were characterized by the loss of microvilli in the renal tubule tells, irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with activated charcoal were similar to those of the control group. In conclusion, activated charcoal may protect the lead-induced toxicity on kidney.

본 연구는 급성 납 독성에 대한 활성탄의 방어효과를 알아보기 위하여 시도되었다. 30g 내외의 생쥐를 대상으로 대조군, 납(60mg/kg)투여군, 납 투여후 활성탄(40mg/kg)투여군으로 구분하여 구강투여 한 후 신장 손상억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액 중 blood urea nitrogen(BUN) 그리고 creatinine (Cre)의 활성도는 활성탄 투여군이 납 투여군보다 감소되었다. 전자현미경적 관찰 결과, 신장의 근위세뇨관에서 납 투여군은 미세융모의 소실이 나타나고, 핵이 함입되어 불규칙해졌으며, 미토콘드리아는 팽대되고, 그 수가 감소하였다. rER의 수조가 확장되고 공포화 현상이 관찰되었으며 리보솜의 탈락이 관찰되었다. 활성탄 투여군은 핵은 거의 원형의 상태이고, 다수의 미토콘드리아가 관찰되었으며, rER은 리보솜이 부착된 상태로 관찰되었다. 이상과 같은 결과, 활성탄이 납 중독된 생쥐에서 납을 흡착시킴으로서 손상된 신장에 보호 효과가 있는 것으로 사료된다.

Keywords

References

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