Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Dose-dependent Ultrastructural Changes of the Odontoblasts in the Neonatal Rat after Ingestion of the Fluoride to the Pregnant Rat

음용수 불소농도에 따른 흰쥐태아 상아모세포의 미세구조적 변화

  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Lim, Do-Seon (Department of Dental Hygiene, Seoul Health College)
  • 정문진 (조선대학교 치과대학 구강조직학교실) ;
  • 정순정 (조선대학교 치과대학 구강조직학교실) ;
  • 임도선 (서울보건대학 치위생과)
  • Published : 2006.03.30
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Abstract

The pregnant rats were given a drinking water administration of the sodium fluoride and normal saline for control animals. The sodium fluoride produced cellular changes of odontoblast with consistent response. Compare to control group, the odontoblasts that were administrated by sodium fluoride, showed significantly ultrastructural differences including large number of free ribosomes and swelled mitochondria in dose-dependent manner (300 ppm). From fine structural and morphological investigations of the changes in odontoblast, there were three distinctive structural changes: (1) destruction of the endoplasmic reticulum, (2) swelling of the mitochondria, and (3) severe cellular derangement of the endoplasmic reticulum and mitochondria. From this consecutive structural change, we observed that sodium fluoride temporarily affects the cell organelles in odontoblasts (100, 200 ppm), suggesting it is important that optimal concentration of the sodium fluoride in developing fetus of the rat.

음용불소가 흰쥐태아의 상아모세포에 간접적으로 미치는 영향과 농도를 확인하기 위해 임신한 흰쥐에 100, 200, 300ppm의 불소가 함유된 음용수를 임신기간 동안 투여하였다. 대조군과 비교하였을 때, 농도에 의존적으로 상아모세포의 미세구조적 변화를 관찰 할 수 있었다. 조면소포체로 부터 리보솜의 탈락과 붕괴, 미토콘드리아의 팽창, 그리고 조면소포체와 미토콘드리아의 변성. 음용 불소의 농도에 따른 미세구조적인 변화로부터 100, 200ppm에서는 가역적인 그리고 300ppm에서는 비가역적인 손상이 유도되는 것을 확인하였다. 또한, 이러한 결과로 미루어 200ppm미만의 농도가 발생중인 흰쥐태아에 영향을 주지 않는 것을 알 수 있다.

Keywords

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