The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Determination of Aroma Components in Pinus densiflora (Pine Needles) Studied by Using Different Extraction Methods

추출방법에 따른 솔잎의 휘발성 성분 조성 비교

  • Published : 2006.06.01
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Abstract

The efficiency of six different extraction methods for the analysis of aroma components from pine needle(P. densiflora) was compared by gas chromatography-mass selective detector(GC-MSD). The six methods were dynamic headspace(DHS), reduced pressure headspace(RPHS), solid-phase microextraction(SPME), simultaneous distillation-extraction(SDE), supercritical fluid extraction(SFE) and pyrolysis distillation extraction(PDE). A total of 65 compounds were identified by using the six different extraction methods. These compounds are classified into six categories in terms of chemical functionality: 25 hydrocarbons, 16 alcohols, 9 carbonyls, 6 esters, 7 acids, and 2 ethers. The aroma compounds having low boiling point were more abundant in DHS, RPHS, and SPME extracts. On the other hand, the aroma compounds having high boiling point were more abundants in SDE, SFE and PDE extracts. The acid compounds were extracted by heat-based extraction methods such as SDE, SFE, PDE, but not by DHS, RPHS and SPME, which used neither solvent nor heat. The oxygenated terpens, hexanal, hexanol, and hexadienal were more abundant in DHS and RPHS extracts, compared with the other methods.

식물체의 향기성분을 분리하는데 주로 사용되는 headspace(DHS, RPHS)법, SPME법, SDE법, SFE법과 최근에 개발된 PDE법으로 솔잎의 휘발성 성분을 추출하였다. 추출된 성분을 Ge/MS를 이용하여 총 65종의 휘발성 성분을 확인하였다. 확인된 성분은 terpene류 25종, alcoholfb류 16종, carbonyls류 9종, esters류 6종, acids류 7종, esters 화합물이 2종인 것으로 나타났다. Headspace(DHS, RPHS)법과 SPME 법에서는 휘발성이 강한 ethyl acetate, 2-ethyl furan, $\beta$-myrcene 등과 같은 저비점 휘발성 성분들이 많이 추출되었다. 반면에, 고비점 휘발성 성분들인 nerolidol, spathulenol, benzoic acid 등은 휘발 성분 추출시 열이 가해지는 SDE법, SFE법, PDE법 등에서는 확인되었으나 headspace법과 SPME법에서는 확인되지 않았다. 이들 6가지 추출방법에서 저비점 휘발성 성분 분석에는 headspace(DHS)법이 가장 좋은 것으로 나타났으며, 고비점 휘발성 성분 분석에는 SDE법이 가장 적합한 것으로 나타났다. 최근에 개발된 PDE법은 SDE법에 비해 추출효율 변에서 약간 떨어지나 전처리 방법이 간단하므로 짧은 시간에 많은 시료를 분석할 때 가장 적합한 방법인 것으로 판단된다.

Keywords

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