Genomics & Informatics

Korea Genome Organization (한국유전체학회)
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Single and Dual Ligand Effects on Gene Expression Changes in Mouse Macrophage Cells

  • Choi Sang-Dun (Department of Molecular Science and Technology, Ajou University) ;
  • Seo Jeong-Sun (Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine)
  • Published : 2006.06.01
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Abstract

We identified differentially expressed genes in RAW264.7 cells in response to single and double ligand treatments (LPS, $IFN{\gamma}$, 2MA, LPS plus $IFN{\gamma}$, and LPS plus 2MA). The majority of the regulated transcripts responded additively to dual ligand treatment. However, a significant fraction responded in a non-additive fashion. Several cytokines showing non-additive transcriptional responses to dual ligand treatment also showed non-additive protein production/secretion responses in separately performed experiments. Many of the genes with non-additive responses to LPS plus 2MA showed enhanced responses and encoded pro-inflammatory proteins. LPS plus $IFN{\gamma}$ appeared to induce both non-additive enhancement and non-additive attenuation of gene expression. The affected genes were associated with a variety of biological functions. These experiments reveal both dependent and independent regulatory pathways and point out the specific nature of the regulatory interactions.

Keywords

References

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