Screening for Fermentative Microorganisms that Grow on Brown Rice with High Amylase and Pretense Activities

Amylase와 Protease의 활성이 높은 현미 발효 미생물의 선별

  • Kim Ki-Yeon (Department of Biotechnology, Chung-Ang University) ;
  • Kim Hee-Gyu (Department of Biotechnology, Chung-Ang University) ;
  • Song Byeong-Chul (Department of Biotechnology, Chung-Ang University) ;
  • Cha Chang-Jun (Department of Biotechnology, Chung-Ang University)
  • 김기연 (중앙대학교 산업과학대학 생명공학과) ;
  • 김희규 (중앙대학교 산업과학대학 생명공학과) ;
  • 송병철 (중앙대학교 산업과학대학 생명공학과) ;
  • 차창준 (중앙대학교 산업과학대학 생명공학과)
  • Published : 2006.06.01

Abstract

Brown rice contains rice bran and germ with higher nutritional value and dietary fiber content compared with the polished rice. However, brown rice has a limitation of poor digestion. fermented brown rice could be better nutritional source and improve digestibility. Therefore, we tried to select good fermentative microorganisms which have nutritional values with high amylase and protease activities, and probiotic effects. Nineteen micro-organisms, including eight Bacillus strains isolated from Chongkukjang and 11 lactic acid bacteria, were screened for the fermentation ability and enzyme production. The liquid broths containing 2.5%(w/v) of raw brown rice powder as a sole nutritional source were used for culture media. Among the strains tested, all of the Bacillus strains and two lactic acid bacteria (Leuconostoc gelidum and Pediococcus pentosaceus) showed increase in cell population and enzyme activities. The viable cell counts of all the Bacillus strains and two lactic acid bacteria exceeded $10^7 CFU/mL$. The maximal enzyme activities produced by Bacillus sp. Bl, Bacillus sp. B2, Bacillus sp. B11, L. gelidum and P. pentosaceus were 17.85, 17.50, 17.10, 17.10 and 3.24 U/mL for amylase and 22.48, 22.04, 23.76, 12.13, and 3.4 U/mL for pretense, respectively. Therefore, the results of this study demonstrated that the above strains could be potential starters for the fermentation of raw brown rice.

현미는 백미에 비하여 식품영양학적 가치 및 식이섬유의 함유량이 높은 쌀겨 및 배아를 포함하고 있으나 소화가 잘되지 않는 단점 이 있다. 따라서 발효현미는 소화력을 높임과 동시에 좋은 영양 공급원이 될 수 있으므로 높은 amylase와 pretense의 활성으로 현미를 발효할 수 있는 미생물을 선별하였다. 2.5%(w/v)의 현미분말을 유일한 영양원으로 한 액체배지를 생장배지로 사용하여 생장능력과 효소 생산능력을 조사하였다. 조사한 8종의 Bacillus 와 11종의 유산균 중에서 모든 Bacillus 균주와 두 종의 유산균이 생장과 효소활성을 보였다. 생균수는 $10^7CFU/mL$을 초과하였으며 Bacillus sp. Bacillus sp. B2, Bacillus sp. B11, Leuconostoc gelidum, Pediococcus pentosaceus 가 생산하는 최고 amylase 활성은 각각 17.85, 17.50, 17.10, 17.10, 3.24 U/ml이었고, 최고 pretense 활성은 각각 22.48, 22.04, 23.76, 12.13, 3.80 U/ml이었다. 따라서 이 균주들은 발효 현미 제조를 위한 접종균주로서 이용이 가능하리라 사료된다.

Keywords

References

  1. Basma, G., S.K. Alya, and M. Nasri. 2003. Stability studies of protease from Bacillus cereus BG1. Enzyme Microb. Technol. 32, 513-518 https://doi.org/10.1016/S0141-0229(03)00004-8
  2. Claus, D. and R.C.W. Berkeley. 1872. Genus Bacillus cohn, p. 1105-1139. In P.H.A. Sneath (ed.), Bergey's manual of systematic bacteriology. The Williams & Wilkins Co., Baltimore, Maryland
  3. Fuller, R. 1989. Probiotic in man and animals. J. Appl. Bactriol. 66, 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  4. Jeoun, K.S., Y.J. Kim, and S.I. Park. 1995. Preparation and characteristics of yogurt from milk added with soy milk and brown rice. Korean J. Food Sci. Technol. 27, 47-55
  5. Kiers, J.L., A.E.A. Van Laeken, F.M. Rombouts, and M.J.R. Nout. 2000. In vitro digestibility of Bacillus fermented soya bean. Int. J. Food Microbiol. 60, 136-169
  6. Lee, H.J., S.M. Byun, and H.S. Kim. 1988. Studies on the dietary fiber of brown rice and milled rice. Korean J. Food Sci. Technol. 20, 576-584
  7. Lee, W.J. and S.S. Kim. 1998. Preparation of Sikhe with brown rice. Korean J. Food Sci. Technol. 30, 146-150
  8. Margosch, D., M.A. Ehrmann, R. Buckow, V. Heinz, R.F. Vogel, and M.G. Ganzle. 2006. High-pressure-mediated survival of Clostridium botulinum and Bacillus amyloliquefaciens endospores at high temperature. Appl. Environ. Microbiol. 72, 3476-3481 https://doi.org/10.1128/AEM.72.5.3476-3481.2006
  9. Mok, C., J. Han, Y.J. Kim, N. Kim, D.Y. Kown, and Y.J. Nam. 1991. Lactic acid fermentation of rice and quality improvement by amylolytic enzyme treatment during fermentation. Korean J. Food Sci. Technol. 23, 739-744
  10. Park, K.B. and S.H. Oh. 2005. Production and characterization of GABA rice yogurt. Food Sci. Biotechnol. 14, 518-522
  11. Saxena, R.K., K. Dutt, L. Agarwal, and R. Nayyar. 2006. A highly thermostable and alkaline amylase from Bacillus sp. PN5. Bioresour. Technol. 1-6
  12. Takasuga, T., K. Senthilkumar, H. Takemori, E. Ohi, H. Tsuji, and J. Nagayama. 2004. Impact of fermented brown rice with Aspergillus oryzae (FEBRA) intake and concentration of polybrominated diphenylethers (PBDEs) in blood of humans from Japan. Chemosphere 57, 759-811
  13. Yoon, S.H. and S.K. Kim. 2004. Physicochemical properties of rice differing in milling degrees. Food Sci. Biotechnol. 13, 57-62