Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Development of a toxA Gene Knock-out Mutant of Pasteurella multocida and Evaluation of its Protective Effects

  • Kim Tae-Jung (School of Veterinary and Biomedical Sciences, Murdoch University) ;
  • Lee Jae-Il (College of Veterinary Medicine, Chonnam National University) ;
  • Lee Bong-Joo (College of Veterinary Medicine, Chonnam National University)
  • Published : 2006.06.01
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Abstract

Pasteurella multocida is an important veterinary and opportunistic human pathogen. In particular, strains of P. multocida serogroup D cause progressive atrophic rhinitis, and produce a potent, intracellular, mitogenic toxin known as P. multocida toxin (PMT), which is encoded by the toxA gene. To further investigate the toxigenic and pathogenic effects of PMT, a toxA-deleted mutant was developed by homologous gene recombination. When administrated to mice, the toxigenicity of the toxA mutant P. multocida was drastically reduced, suggesting that the PMT constributes the major part of the toxigenicity of P, multocida. Similar results were obtained in a subsequent experiment, while high mortalities were observed when toxA(+) P. multocida bacterial culture or culture Iysate were administrated. Mice immunized with toxA(-) P. multocida were not protected (none survived) following challenge with toxA(+) P. multocida or bacterial culture Iysate (toxin). These results suggest that the toxigenicity of P. multocida is mainly derived from PMT.

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References

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