Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Difference in Protein Markers According to the Survival of Sepsis Patients using Protein Chips

패혈증 생존 및 사망 환자 혈장에서 단백질 칩을 이용한 분석의 차이

  • Park, Myoung Ok (Department of Internal Medicine, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Lee, Heui Young (Department of Internal Medicine, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Son, Hee Jung (Department of Anesthesia, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Sung, Ji Hyun (Department of Molecular and Cellular Biochemistry, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Lee, Seung Joon (Department of Internal Medicine, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Lee, Sung Joon (Department of Internal Medicine, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Ha, Kwon Soo (Department of Molecular and Cellular Biochemistry, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital) ;
  • Kim, Woo Jin (Department of Internal Medicine, College of Medicine, Kangwon National University and the Clinical Research Institute of Kangwon National University Hospital)
  • 박명옥 (강원대학교 의과대학 내과학교실 및 강원대학교병원 임상의학연구소) ;
  • 이희영 (강원대학교 의과대학 내과학교실 및 강원대학교병원 임상의학연구소) ;
  • 손희정 (강원대학교 의과대학 마취과학교실 및 강원대학교병원 임상의학연구소) ;
  • 성지현 (강원대학교 의과대학 생화학교실 및 강원대학교병원 임상의학연구소) ;
  • 이승준 (강원대학교 의과대학 내과학교실 및 강원대학교병원 임상의학연구소) ;
  • 이성준 (강원대학교 의과대학 내과학교실 및 강원대학교병원 임상의학연구소) ;
  • 하권수 (강원대학교 의과대학 생화학교실 및 강원대학교병원 임상의학연구소) ;
  • 김우진 (강원대학교 의과대학 내과학교실 및 강원대학교병원 임상의학연구소)
  • Received : 2006.03.22
  • Accepted : 2006.07.10
  • Published : 2006.07.30
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Abstract

Background; Several clinical scoring systems are currently being used to predict the outcome of sepsis, but they all have certain limitations. Therefore, we sought to identify the proteomic biomarkers, with wsing proteomic tools, that differed according to the outcome of sepsis patients. Methods; Upon admission to the ICU, blood samples were obtained from the 16 patients with sepsis who were enrolled in this study. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI -TOF MS) was used to identify the markers that could predict the outcome of sepsis. Results; We found six peaks, by using cation and anion chips, that statistically differed between those patients who died and those who survived. Conclusion; The biomarkers we found by using proteomic tools may help predict the prognosis and also plan the treatment of sepsis.

배 경: 패혈증 환자의 예후를 예측하는 데 현재 사용되고 있는 임상적 채점 방식은 몇가지 제한점이 있다. 그래서 단백질체학(proteomics) 기법을 사용하여 표지자(proteomic biomarkers)를 찾으려 연구를 진행하였다. 방 법: 본 연구에서는 16명의 패혈증환자에게서 중환자실에 입원하자마자 혈장을 채취하였다. 패혈증의 예후를 예측할 수 있는 표지자를 찾기 위해 Surface-enhanced laser desorption/ionization time-of-flight (SELDI -TOF) mass spectrometry를 사용하였다. 결 과: 사망환자와 생존환자 사이에 통계적으로 유의한 차이가 있는 6개의 단백표지자를 발견하였고 이들은 패혈증 환자의 예후 예측과 치료계획수립에 도움이 될 것으로 생각된다. 결 론: 프로테오믹 마커는 패혈증 환자의 예후를 예측하고 치료계획을 세우는 데 있어 유용하게 이용될 가능성이 있을 것으로 사료된다.

Keywords

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