Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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An Auxin Producing Plant Growth Promoting Rhizobacterium Bacillus subtilis AH18 which has Siderophore-Producing Biocontrol Activity

Auxin과 Siderophore 생산성 다기능 생물방제균 Bacillus subtilis AH18

  • Jung Hee-Kyoung (Department of Applied Microbiology, College of Natural Resources, Yeungnam University) ;
  • Kim Jin-Rak (Department of Applied Microbiology, College of Natural Resources, Yeungnam University) ;
  • Woo Sang-Min (Department of Applied Microbiology, College of Natural Resources, Yeungnam University) ;
  • Kim Sang-Dal (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
  • 정희경 (영남대학교 자연자원대학 응용미생물학과) ;
  • 김진락 (영남대학교 자연자원대학 응용미생물학과) ;
  • 우상민 (영남대학교 자연자원대학 응용미생물학과) ;
  • 김상달 (영남대학교 자연자원대학 응용미생물학과)
  • Published : 2006.06.01
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Abstract

To isolate a bacterium that produces plant growth promoting hormone, a total of 29 bacteria were obtained from the soil in Gyeongsan, Korea. Among these, 14 strains were selected by their positive reaction on Salkowski to produce auxin. All of these were then tested for their property to produce siderophore using CAS (chrome azurol S) blue agar, and one was chosen for its ability to produce both, auxin and siderophore. This strain, denoted, AHl8, showed 1.5 times higher adventitious root induction rates than controls, using mung-beans. The strain also showed efficient biocontrol properties towards Fusarium-wilt of tomatoes in artificial pot assays. The strain was identified as Bacillus subtilis by 16s rDNA comparison and Biolog analyses. Growth and media conditions for Bacillus subtilis AH1 8 to highly produce siderophore were also investigated.

식물생장 촉진 호르몬인 auxin을 생산하는 균주를 선발하기 위해 경산지역 경작지 토양에서 29종의 세균을 분리하였으며 Salkowsky test를 통해 auxin을 생산하는 14종을 선발하였다. 이 중 CAS(chrome azurol S) blue agar에서 siderophore의 생산을 확인 후 auxin과 siderohroe를 동시에 생산하는 AH18을 최종 선발하였다. AH18의 mung-bean adventitious root induction test를 통해 식물생장 촉진 능이 대조구에 비해 1.5배나 뛰어남을 알 수 있었고, 토마토 pot test에서 토마토 시들음병의 윈인균인 F. oxysporum에 대해서 길항력도 나타내었다. 선발된 AHl8 균주는 16S rDNA와 Biolog system을 통해 Bacillus subtilis 동정되었다. B. subtilis AH18은 Sucrose-asparagine-MgsO$_4$(SAM) medium (pH 6.0)에 접종하여 30$^{\circ}C$에서 3일간 배양 시 siderophore를 가장 많이 생산하는 것을 알 수 있었다.

Keywords

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