Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

In vitro Regeneration of Phragmites australis through Embryogenic Cultures

  • Lee Jeong-Sun (Division of Natural Sciences, Ajou University) ;
  • Kim Chang-Kyun (Center for Fungi and Plants Genome Research, FnP Corp., Biovalley #206, Seoul National University) ;
  • Kim In-Sung (School of Biological Sciences, Seoul National University) ;
  • Lee Eun-Ju (School of Biological Sciences, Seoul National University) ;
  • Choi Hong-Keun (Division of Natural Sciences, Ajou University)
  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Phragmites australis (reed) has received much attention as being one of the principle emergent aquatic plants for treating industrial and civil wastewater. Plant regeneration via plant tissue culture in p. australis was investigated. Three types of callus were identified from seeds on N6 medium plus 4.5 UM 2,4-dichlorophenoxyacetic acid (2,4-D). Yellow compact type showed the best redifferentiation, whereas white compact type and yellow friable were not competent to differentiate into plane. Solid medium culture was better than liquid suspension culture for enhancing callus growth when N6 medium supplemented with 4.5 ${\mu}M$ 2,4-D was used. Phytagel, as a gelling agent, was superior to agar in plant regeneration on N6 medium, supplemented with 9.4 ${\mu}M$ kinetin and 0.54 ${\mu}M$ $\alpha$-naphthaleneacetic acid (NAA). Transfer of the plantlets regenerated from kinetin and NAA-supplemented N6 medium to growth regulator-free MS medium enhanced the further development of the plantlets. Plantlets on subsequently grown to maturity when tansferred to potting soil. The regenerated plants exhibited morphologically normal. The system for plant regeneration of P. australis enables to propagate elite lines on a large scale for water purification in the ecosystem

Keywords

References

  1. Cho MC, Juang UD, Park SG, Park Y (2003) Regeneration and acclimatization of plants derived from anther cultures in carrot (Daucus carota L.). Kor J Plant Biotechnology 30: 47-52
  2. Choi H.-K. (2000) Aquatic Vascular Plants. A series of Korean Plants 5. Jungheng Publishers, Seoul, pp 9-13
  3. Cooper PF, Boon AG (1987) The use of Phragmites for wastewater treatment by the root zone method: The UK approach. In: Reddy KR, Smith WH (eds), Aquatic Plants for Water Treatment and Resource Recovery, Mangnolia Pub Inc, Orlando, Florida, pp 153-174
  4. Duncan DB (1955) Multiple range and multiple F test. Biometrics 11: 1-42 https://doi.org/10.2307/3001478
  5. Gersberg RM, Elkins BV, Lyons RS, Goldman CR (1986) Role of aquatic plants in wastewater treatment by artificial wetlands. Water Research 20: 363-368 https://doi.org/10.1016/0043-1354(86)90085-0
  6. Gray NF (1989) Biology of Wastewater Treatment. Oxford Univ Press, pp 828
  7. Hammer DA (1996) Creating Freshwater Wetlands. Lewis Publisher, New York, pp 406
  8. Kenneth RG, Biddlestone AJ (1995) Engineered reed-bed systems for wastewater treatment. Trend Biotechnol 13: 248-252 https://doi.org/10.1016/S0167-7799(00)88958-1
  9. Krishnaraj S, Vasjl IK (1995) Somatic embryogenesis in herbaceous monocots. In: Thorpe TA (ed) In Vitro Embryogenesis in Plant. Kluwer Academic Press, Dordrecht, pp 417-470
  10. Larkin PJ, Scowcroft WR (1981) Somaclonal variation-a novel source for variability from cell culture for plant improvement. Theor Appl Genet 60: 197-214 https://doi.org/10.1007/BF02342540
  11. Lauzer D, Dallaire S, Vincent G (2000) In vitro propagation of reed grass by somatic embryogenesis. Plant Cell Tiss Org Cult 60: 229-234 https://doi.org/10.1023/A:1006499419126
  12. Lee HY, Jeon JO, No JG, Park HG (1999) Effects of several factors on callus induction in anther culture of cherry tomato. J Kor Soc Hort Sci 40: 537-540
  13. Lee S, Jeon JS, Jung KH, An GH (1999) Binary vectors for efficient transformation of rice. J Plant Bioi 42: 310-316 https://doi.org/10.1007/BF03030346
  14. Mathe C, Hamvas MM, Grigorszky I, Vasas G, Molnar E, Power JB, Davey MR, Borbely G(2000) Plant regeneration from embryogenic cultures of Phragmites australis (Cav.) Trin. ex Steud. Plant Cell Tiss Org Cult 63: 81-84 https://doi.org/10.1023/A:1006471515103
  15. McGranahan G, Leslie CA, Driver JA (1988) In vitro propagation of mature Persian walnut cultivars, HortSci 53: 63-72
  16. Negri MC, Hinchman RR (1996) Plants that remove contaminants from the environment. Laboratory Medicine 27: 36-40 https://doi.org/10.1093/labmed/27.1.36
  17. Nichols DS (1983) Capacity of natural wetlands to remove nutrients from wastewater. Res Jour WPCF 55: 495-505
  18. Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): Evidence for somatic embryogenesis. Protoplasma 110: 95-105 https://doi.org/10.1007/BF01281535
  19. Park MC, Shin JS, Kim NR, Cho HJ, Cho HJ, Park SH, An KS, Lee SC, An GH (2003) High-frequency agrobacteriummediated genetic transformation of Tongil rice varieties. J Plant Biol 46: 23-30 https://doi.org/10.1007/BF03030297
  20. Park SY, Kim SD, Sin SK, Lee CH, Paek KY (1997) Several factors on bulblets regeneration from callus culture in Uilium longiflorum 'Gelia'. Kor J Plant Tiss Cult 24: 183-188
  21. Pasqualetto PL, Zimmerman RH, Fordham I (1986) Gelling agent and growth regulator effects on shoot vitrification of 'Gala' apple in vitro. J Am Soc Hort Sci 111: 976-980
  22. Sangwan RS, Gorenflot R (1975) In vitro culture of Phragmites tissue: Callus formation, organ differentiation and cell suspension culture. Z PfIanzenphysiol Bd 75 S: 256-269
  23. Straub PF, Decker DM, Gallagher JL (1988) Tissue culture and long-term regeneration of Phragmites australis (Cav.) Trin. ex Steud. Plant Cell Tiss Org Cult 15: 73-78 https://doi.org/10.1007/BF00039891
  24. Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J Plant Physiol 128: 193-218 https://doi.org/10.1016/S0176-1617(87)80234-1
  25. Vasil V, Vasil IK (1984) Induction and maintenance of embryogenic callus cultures of Gramineae. In: Vasil IK (ed), Cell Culture and Somatic Cell Genetics of Plants. Academic Press, New York, pp 36-42
  26. Wang J, Seliskar DM, Gallagher JL (2004) Plant regeneration via somatic embryogenesis in the brackish wetland monocot Scirpus rubustus. Aquatic Botany 79: 163-174 https://doi.org/10.1016/j.aquabot.2004.02.003
  27. Wang W, Cui SX, Zhang CL (2001) Plant regeneration from embryogenic suspension cultures of dune reed. Plant Cell Tiss Org Cult 67: 11-17 https://doi.org/10.1023/A:1011634827298
  28. Yang YG, Guo YM, Guo Y, Guo ZC, Lin JX (2003) Regeneration and large-scale propagation of Phragmites communis through somatic embryogenesis. Plant Cell Tiss Org Cult 75: 287-290 https://doi.org/10.1023/A:1025871015405