생명과학회지 (Journal of Life Science)

  • 제16권3호
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  • Pages.442-446
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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참죽나무에서 분리한 flavonol glycoside의 금속단백분해효소-2 억제 활성

Inhibition of Matrix Metalloproteinase-2 Activity of Flavonol Glycosides from Cedreia sinensis

  • 황선우 (경상대학교 응용생명과학과) ;
  • 하태정 (영남농업연구소) ;
  • 고영희 (한국생명공학연구원) ;
  • 전효곤 (한국생명공학연구원) ;
  • 이준 (경상대학교 응용생명과학과) ;
  • 권현숙 (경상대학교 응용생명과학과) ;
  • 박기훈 (경상대학교 응용생명과학과) ;
  • 양민석 (경상대학교 응용생명과학과)
  • Hwang Seon-Woo (Division of Applied Life Science, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Ha Tae-Joung (Yeongnam Agricultural Research Institute, National Institute of Crop Science (RDA)) ;
  • Kho Yung-Hee (Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Chun Hyo-Kon (Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Lee Jun (Division of Applied Life Science, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Kwon Hyun-Sook (Division of Applied Life Science, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Park Ki-Hun (Division of Applied Life Science, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Yang Min-Suk (Division of Applied Life Science, Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 발행 : 2006.06.01
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초록

참죽나무는 멀구슬과 나무로서 한국과 중국에 널리 분포하고 있다. 한방에서는 장염(腸炎), 이질(痢疾), 개선(疥癬) 등의 치료에 이용되고 있다. 본 연구는 참죽나무 잎에서 3종의 flavonol glycosides를 분리하여 NMR을 통해 구조를 확인한 결과, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2)와 quercetin-3-O-glucoside(3)로 구조 동정되었다. 분리된 화합물들의 생리활성은 matrix metalloproteinase-2 저해 활성과 인체암 세포주에 대한 세포독성과 apoptosis실험을 통하여 항암효과를 조사하였다. 그 결과 인체암 세포주에 대한 세포독성과 apoptosis 저해활성은 나타내지 않았으나, MMP-2활성저해 조사에서는 강한 저해 효과를 나타내었다. 특히 quercetin-3-O-glucoside는 암세포에 직접적인 독성을 보이는 것이 아니라 암의 침윤과 전이에 특이적으로 작용하는 물질로 보여진다.

Cedrela sinensis is a broadleaf tree that is widely cultivated in Korea and China. It was used for treating enteritis, dysentery, and skin itch in oriental medicine. In this study, three major flavonoids, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2), and quercetin-3-O-glucoside (3), were isolated from the leaf of Cedrela sinensis. The biological activities of these compounds were tested by inhibitory activity of matrix metalloproteinases-2 (Type IV collagenase) method together with a cytotoxicity and a apoptosis test against human cancer cell lines.

키워드

참고문헌

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피인용 문헌

  1. Stimulation of Activity and Expression of Antioxidant Enzymes by Solvent Fractions and Isolated Compound fromCedrela sinensisLeaves in HepG2 Cells vol.14, pp.4, 2011, https://doi.org/10.1089/jmf.2010.1213