Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Chemopreventive Potential of Salvia miltiorrhiza Fraction Extracts

단삼 분획추출물의 암예방 효과

  • Shon Yun-Hee (College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Cho Hyun-Jung (College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Chang Hyeun-Wook (College of Pharmacy, Yeungnam University) ;
  • Son Kun-Ho (College of Human Ecology and Kinesiology, Andong National University) ;
  • Nam Kyung-Soo (College of Medicine and Intractable Disease Research Center, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실) ;
  • 조현정 (동국대학교 의과대학 약리학교실) ;
  • 장현욱 (영남대학교 약학대학) ;
  • 손건호 (안동대학교 생활과학대학 및 난치병한양방치료연구소) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Published : 2006.06.01
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Abstract

Six fractions of Salvia miltiorrhiza were tested for their chemopreventive potentials using biochemical markers of carcinogenesis such as quinone reductase (QR), glutathione S-transferase (GST) and glutathione (GSH). Seventy percentage of EtOH extract was potent inducer of QR activity in Hepa1c1c7 murine hepatoma cells. GST activity was increased about 1.4-fold with EtOAc extract at concentration of 50 ${\mu}g/ml$. GSH levels were significantly increased with $H_2O$ extract, 70% EtOH extract and water extract at concentration of 50 ${\mu}g/ml$ (p<0.005). From these results, 70% EtOH extract (250 mg/kg) was intragastrically administered to ICR mice for 14 days. QR, GST and GSH levels were significantly increased with the 70% EtOH treatment. These studies suggest that the 70% EtOH extract of S. miltiorrhiza could be considered as a potential agent for cancer chemoprevention.

본 연구는 단삼 분획추출물로부터 in vitro와 in vivo상에서 QR과 GST의 활성 유도와 GSH의 함량변화를 지표로 암예방 효과를 측정하였다. Hepalcla7 세포에 대한 in vitro상에서의 실험결과 QR 활성 유도율은 70% EtOH 추출물 50 ${\mu}g/ml$ 처리군에서 2.5배로 가장 높은 유도율을 나타내었고, GST 활성 측정은 EtOAc추출물 50 ${\mu}g/ml$농도에서 1.4배의 유도율을 나타내었다. GSH 생성변화를 살펴본 결과에서는 $H_2O$추출물, 70% EtOH 추출물 그리고 water layer 추출물 50 ${\mu}g/ml$ 농도에서 높은 생성율을 나타내었다. 이상의 결과에서 QR활성과 GSH 함량변화에서 높은 증가효과를 나타낸 70% EtOH 추출물을 관류법으로 마우스에 투여하여 in vivo 상에서의 QR과 GST의 활성 변화와 GSH 함량을 측정한 결과 QR, GST활성과 GSH함량이 250 mg 투여시 각각 1.7배 및 1.5배의 활성 증가와 1.4배 함량증가를 측정할 수 있었음으로 70% EtOH추출물은 암예방효과가 가장 높은 것으로 생각된다.

Keywords

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