환경생물 (Korean Journal of Environmental Biology)

  • 제24권2호
  • /
  • Pages.195-200
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  • 2006
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지

Formalin에 노출시킨 넙치 (Paralichthys olivaceus) 간장 약물대사효소의 in vivo 및 in vitro 반응

In vitro and In vivo Responses of Hepatic Xenobiotic Metabolizing Enzymes in Flounder (Paralichthys olivaceus) Exposed to Formalin

  • 이지선 (강릉대학교 해양생명공학부/동해안해양생물자원연구센터) ;
  • 하진환 (제주대학교 식품생명공학과) ;
  • 이경선 (목포해양대학교 해양환경공학) ;
  • 전중균 (강릉대학교 해양생명공학부/동해안해양생물자원연구센터)
  • Lee, Ji-Seon (Division of Marine Bioscience and Technology, Kangnung National University/EMBRC) ;
  • Ha, Jin-Hwan (College of Engineering, Cheju National University) ;
  • Lee, Kyoung-Seon (Division of Ocean System Engineering, Mokpo National Maritime University) ;
  • Jeon, Joong-Kyun (Division of Marine Bioscience and Technology, Kangnung National University/EMBRC)
  • 발행 : 2006.06.01
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초록

The response of hepatic mixed function oxygenase (MFO) system was investigated in olive flounder exposed to formalin. Hepatic microsome of olive flounder incubated in vitro with formalin demonstrated the induction of cytochrome P450 (CYP), ethoxyresorufin deethylase (EROD), cytochrome P450 reductase (P450R) and cytochrome b5 reductase (b5R) activity. In addition, olive flounder was exposed to 100, 300 and 500 ppm of formalin for 1 h and then transferred to a flow-through type of 1000 L aquarium. Hepatic MFO enzyme activity was determined for 72 h. As the result, hepatic CYP, P450R and EROD activities increased following exposure of formalin, but b5R and GST showed no significant change. These results imply that CYP and P450R can be considered as main hepatic enzymes involving in detoxification of formalin.

키워드

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