Differential Expression of C4H and F5H Genes in Rice (Oryza sativa L.) after Gamma-irradiation

  • Park, Young-Mi (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Chae, Hyo-Seok (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Chung, Byung-Yeoup (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Kim, Jae-Sung (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Kim, Jin-Hong (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Wi, Seung-Gon (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • An, Byung-Chull (Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Cho, Jae-Young (Division of Biological Resources Sciences, Chonbuk National University)
  • 발행 : 2006.06.01

초록

To reveal effects of gamma-irradiation with various doses on the expressions of C4H and F5H genes, the transcription levels of OsC4HL and OsF5HL were investigated in leaves and stems of two rice cultivars, Ilpoombyeo and IR-29, after the irradiation with 5, 10, 50, or 100 Gy for 4 h. In overall pattern of 24 h after the irradiation, the transcription levels of the two genes increased with the increasing doses of radiation in the leaves of both cultivars, except that of OsC4HL in IR-29. However, in the stems, the transcription level of OsF5HL increased in Ilpoombyeo and decreased in IR-29 dose-dependently, while that of OsC4HL decreased in Ilpoombyeo with the increasing doses of radiation and remained constant in IR-29. When the expressions of OsC4HL and OsF5HL were investigated in a time-course after the irradiation with 100 Gy, they reached their highest levels in the leaves of both cultivars 5 hand 72 h after the irradiation, respectively. Therefore, we suggest that the expressions of OsC4HL and OsF5HL, which involved in the same phenylpropanoid pathway, are differentially regulated during the post-irradiation period, showing different cultivar and tissue specificity. Furthermore, the dose dependency of the gene expressions is also discussed immediately after the irradiation.

키워드

참고문헌

  1. Calabrese EJ. 2002. Horrnesis: changing view of the dose-response, a personal account of the history and current status. Mutat. Res. 511:181-189 https://doi.org/10.1016/S1383-5742(02)00013-3
  2. Casarett AP. 1968. Radiation chemistry and effects of gamma radiation on the cell. In AP Casarett, ed, Radiation Biology. Prentice-Hall, Englewood Cliffs, NJ
  3. Chapple C, T Vogt, BE Ellis and CR Somerville. 1992. An Arabidopsis mutant defective in the general phenylpropanoid pathway. Plant Cell 4: 1413-1424 https://doi.org/10.1105/tpc.4.11.1413
  4. Chapple C. 1998. Molecular-genetic analysis of plant cytochrome P450-dependent monooxygenases. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49:311-343 https://doi.org/10.1146/annurev.arplant.49.1.311
  5. Cho H-S, H-S Lee and H-S Pai. 2000. Expression patterns of diverse genes in response to gamma irradiation in Nicotiana tabacum. J. Plant Biol. 43:82-87 https://doi.org/10.1007/BF03030499
  6. Day TA. 1993. Relating UV -B radiation screening effectiveness of foliage to absorbing-compound concentration and anatomical characteristics in a diverse group of plants. Oecologia 95:542-550 https://doi.org/10.1007/BF00317439
  7. Frank MR, JM Deyneka and MA Schuler. 1996. Cloning of wound-induced cytochrome P450 monooxygenases expressed in pea. Plant Physiol, 110: 1035-1046 https://doi.org/10.1104/pp.110.3.1035
  8. Gravot AR, A Larbat, K Hehn, E Lievre, JL Gontier, Goergen and F Bourgaud. 2004. Cinnamic acid 4-hydroxylase mechanism-based inactivation by psoralen derivatives: cloning and characterization of a C4H from a psoralen producing plant-Ruta graveolens-exhibiting low sensitivity to psoralen inactivation. Arch. Biochem. Biophys. 422:71-80 https://doi.org/10.1016/j.abb.2003.12.013
  9. Hahlbrock K and D Scheel. 1989. Physiology and molecular biology of phenylpropanoid metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40:347-369 https://doi.org/10.1146/annurev.pp.40.060189.002023
  10. Kim J-H, M-H Baek, BY Chung, YB Lee and J-S Kim. 2004. Comparison of sensitivity to photoinhibition and UV-B stress between developing and mature leaves of red pepper (Capsicum annuum L.) plants from control and gammairradiated seeds. J. Kor. Soc. Hort. Sci. 45:66-73
  11. Kim J-H, DH Yang, J-S Kim, M-H Baek, YM Park, SG Wi, JY Cho, BY Chung. 2006. Cloning, characterization and expression of rice ferulate-5-hydroxylase gene, a cytochrome P450-dependent monooxygenase. J. Plant Biol. (in press) https://doi.org/10.1007/BF03030533
  12. Lee H-Y, J-S Kim, M-H Baek, S-C Park and Y-I Park. 2002. Effects of low dose y-radiation on photosynthesis of red pepper (Capsicum annuum L.) and the reduction of photoinhibition. Korean J. Environ. Agr. 21:83-89 https://doi.org/10.5338/KJEA.2002.21.2.083
  13. Lee H-Y, J-S Kim, M-H Baek, JC Yoo and ST Kwon. 2003. Effects of low dose y-irradiation on the physiological activities of radish (Raphanus sativus L.) during early growth and the reduction of ultraviolet-B stress. J. Kor. Soc. Hort. Sci. 44:314-320
  14. Luckey TD. 1980. Hormesis with ionizing radiation. CRC press, Boca Raton
  15. McLennan AG. 1988. DNA damage, repair, and mutagenesis, In JA Bryant, LD Val gene, eds, DNA replication in plants, CRC Press, Boca Raton, FL, pp 135-186
  16. Meyer K, AM Shirley, JC Cusumano, DA Bell-Lelong and C Chapple. 1998. Lignin monomer composition is determined by the expression of a cytochrome P450-dependent monooxygenase in arabidopsis, Proc. NatI. Acad. Sci. USA 95:6619-6623
  17. Mizutani M, D Ohta and R Sato. 1997. Isolation of a eDNA and a genomic clone encoding cinnamate 4-hydroxylase from arabidopsis and its expression manner in planta, Plant Physiol. 113:755-763 https://doi.org/10.1104/pp.113.3.755
  18. Teutsch HG, MP Hasenfratz, A Lesot, C Stoltz, J Garnier, J Jeltsch, F Durst and D Werck-Reichhart. 1993. Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate-t-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway. Proc. Natl. Acad. Sci. USA 90:4102-4106
  19. Wi SG, BY Chung, J-H Kim, M-H Baek, DH Yang, JW Lee and J-S Kim. 2005. Ultrastructural changes of cell organelles in arabidopsis stems after gamma irradiation. J. Plant Biol. 48:195-200 https://doi.org/10.1007/BF03030408
  20. Yamanouchi UM, H Yano, M Lin, Ashikari and K Yamada. 2002. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc. Natl, Acad. Sci. USA 99:7530-7535
  21. Yang DH, BY Chung, J-S Kim, J-H Kim, PY Yun, YK Lee, YP Lim and MC Lee. 2005a. cDNA cloning and sequence analysis of rice cinnamate-4-hydroxylase gene, a cytochrome P450-dependent monooxygenase, involving in the general phenylpropanoid pathway. J. Plant Biol. 48:311-318 https://doi.org/10.1007/BF03030528