Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Analysis of Differentially Expressed Proteins in Bovine Longissimus Dorsi and Biceps Femoris Muscles

  • Kim, S.M. (Department of Biology, Kyung Hee University) ;
  • Park, M.Y. (Department of Biology, Kyung Hee University) ;
  • Seo, K.S. (Animal Genetic Evaluation Division, National Livestock Research Institute, RDA) ;
  • Yoon, D.H. (Animal Genomics & Bioinformatics Division, National Livestock Research Institute, RDA) ;
  • Lee, H.-G. (School of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Y.J. (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, S.H. (Department of Biology, Kyung Hee University)
  • Received : 2005.11.11
  • Accepted : 2006.04.06
  • Published : 2006.10.01
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Abstract

Skeletal muscle contains slow and fast twitch fibers. These skeletal muscle fibers express type I and type II myosin, respectively, and these myosin isoenzymes have different ATPase activity. The aim of this study was to investigate protein profiles of bovine skeletal muscles by proteomic analysis. Fifty seven spots of distinct proteins were excised and characterized. The expression of sixteen spots was differed in longissimus dorsi muscle with a minimal 2-fold change compared to biceps femoris muscle. The majority of differentially expressed proteins belonged to metabolic regulation-related proteins such as glyceraldehyde 3-phosphate dehydrogenase, triosephosphate isomerase and carbonic anhydrase 3. The real time-PCR assay confirmed an increase or induction of specific genes: RGS12TS isoform, GAPDH, triosephosphate isomerase and carbonic anhydrase. These results suggest that the expression of metabolic proteins is under a specific control system in different bovine skeletal muscle. These observations could have significant implications for understanding the physiological regulation of bovine skeletal muscles.

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References

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