Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association between SNPs within Prolactin Gene and Milk Performance Traits in Holstein Dairy Cattle

  • He, Feng (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Sun, Dongxiao (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Yu, Ying (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Wang, Yachun (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Zhang, Yuan (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University)
  • Received : 2006.01.26
  • Accepted : 2006.03.02
  • Published : 2006.10.01
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Abstract

Prolactin plays an important role in mammary gland development, milk section initiation and maintenance of lactation, so the bovine prolactin gene is considered as a potential quantitative trait locus affecting milk performance traits in dairy cattle. In this study, to determine the association between prolactin and milk performance traits, the genetic polymorphisms of a part of the prolactin gene were detected in a population of 649 cows of Chinese Holstein Dairy Cattle. Three SNPs in the promoter and one SNP in the intron1 of prolactin were identified, which was A/C (-767), G/T (-485), C/A (-247), and C/T (427), respectively. Statistical results indicated that one of SNP within promote, CHBP2, was significantly associated with milk yield (p<0.01), fat yield (p<0.05), protein yield (p<0.01), and protein percentage (p<0.05). The cows with genotype BB of CHBP2 had significantly higher milk yield (p<0.01), fat yield (p<0.05), and protein yield (p<0.01) than those of cows with genotype AA, while cows with genotype AA showed the highest protein percentage (p<0.05). In addition, based on the nine major haplotypes constructed from the four SNPs, the association analysis between diplotypes and milk performance trait was carried out. Results showed that the least square mean for fat yield of diplotype H2H8 was significantly higher than those of other eleven diplotypes (p<0.05). Our findings implied that CHBP2 and H2H8 of prolactin would be useful genetic markers in selection program on milk performance traits in Holstein Dairy Cattle.

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References

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