Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Prevalence of Fimbrial Antigen (K88 variants, K99 and 987P) of Enterotoxigenic Escherichia Coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China

  • Wang, J. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University) ;
  • Jiang, S.W. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University) ;
  • Chen, X.H. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University) ;
  • Liu, Z.L. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University) ;
  • Peng, J. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
  • Received : 2005.07.17
  • Accepted : 2005.12.13
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003, 227 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). Twenty-three (10.1%) of 227 fecal samples were found to contain fimbrial antigen K88, which was identified as K88ac variant; and 13 (5.7%) samples containing K99. In 2004, another 179 fecal samples from diarrheic piglets, 1 day to 6 weeks of age, were tested for prevalence of fimbrial antigen K88, K99 and 987P. Forty-seven (26.3%) of the 179 samples carried at least one of the ETEC fimbrial antigens. K88 antigen was detected in 20.1%. In the 36 samples known to carry fimbrial antigen K88, 32 (88.9%) contained K88ad; and 4 (11.1%) contained K88ac; none of them carried K88ab. Fimbrial antigens K99 and 987P were detected in 1.1% and 6.1%, respectively. Our data indicate that K88 is the most common fimbrial antigen of ETEC associated with diarrhea in piglets in Central China.

Keywords

References

  1. Alexander, T. J. L. 1994. Neonatal diarrhea in pigs. In: (C. L. Gyles) in domestic animals and humans. CAB International, Wallingfird, UK, pp. 151-170
  2. Broeck, W. V. D., E. Cox, B. Oudega and B. M. Goddeeris. 2000. The F4 fimbrial antigen of Escherichia coli and its receptors. Vet. Microbiol. 71:223-244 https://doi.org/10.1016/S0378-1135(99)00174-1
  3. Choi, C. S. and C. H Chae, 1999. Genotypic prevalence of F4 variants (ab, ac, and ad) in Escherichia coli isolated from diarrheic piglets in Korea. Vet. Microbiol. 67:307-310 https://doi.org/10.1016/S0378-1135(99)00046-2
  4. Evans, D. G., D. J. Evans, Jr. and W. Tjoa. 1977. Hemaggluation of human group A erythrocyte by enterotoxigenic Escherichia coli isolated from adults with diarrhea: Correlation with colonization factor. Infect. Immun. 18:330-337
  5. Evelyn, A. D. and E. S. James. 1994. Age-related resistance to 987P fimbria mediated colonization correlates with specific glycolipid receptors in intestinal mucus in swine. Infect. Immun. 62:4789-4794
  6. Evelyn, A. D., C. W. Shannon and H. W. Moon. 1989. Agespecific colonization of porcine intestinal epithelium by 987Ppiliated enterotoxigenic Escherchia coli. Infect. Immun. 57:82-87
  7. Franklin, M. A., D. H. Francies and A. G. Mathew. 1996. A PCRbased method of detection and differentiation of $K88^+$ adhesive Escherichia coli. J. Vet. Dian. Invest. 8:460-463 https://doi.org/10.1177/104063879600800410
  8. Garabal, J. I., F. Vazquez, J. Blanco, M. Blanco and E. A. Gonzalez. 1997. Colonization antigens of enterotoxigenic Escherichia coli strains isolated from piglets in Spain. Vet. Microbiol. 54:321-328 https://doi.org/10.1016/S0378-1135(96)01291-6
  9. Guinee, P. A. M. and W. H. Jansen. 1979. Behavior of Escherichia coli K antigens K88ab, K8ac, and K88ad in immunoelectrophoresis, double diffusion, and hemagglutination. Infect. Immun. 3:700-705
  10. Hampson, D. J. 1994. Post-weaning Escherichia coli diarrhea in pigs. In: (Ed. C. L. Gyles) in domestic animals and humans. CAB International, Wallingfird, UK. pp. 629-647
  11. Harel, J., H. Lapinte, A. Fallara, L. A. Lortie, M. Bigras-Poulin, S. Lariviere and J. M. Fairbrother. 1991. Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea. J. Clin. Microbiol. 29:745-752
  12. Hong, J. W., O. S. Kwon, B. J. Min, W. B. Lee, K. S. Shon, I. H. Kim and J. W. Kim. 2004. Evaluation effects of spray-dried egg protein containing specific egg yolk antibodies as a substitute for spray-dried plasma protein or antibiotics in weaned pigs. Asian-Aust. J. Anim. Sci. 17:1139-1144 https://doi.org/10.5713/ajas.2004.1139
  13. Hua, R. H., S. X. Zhang, K. W. He, Y. X. Ni and Z. Z. Yang. 2002. Detection of 987P positive enterotoxigenic Escherichia coli by polymerase chain reaction. Chi. J. Vet. Sci. Tech. 32:3-5
  14. Jin, L. Z. and X. Zhao. 2000. Intestinal receptors for adhesive fimbriae of enterotoxigenic Escherichia coli (ETEC) K88 in swine - a review. Appl. Microbiol. Biotchnol. 54:311-318 https://doi.org/10.1007/s002530000404
  15. Kwon, D., O. Kim and C. Chae. 1999. Prevalence of genotypes for fimbriae and enterotoxins, and of O serogroups in Escherichia coli isolated from diarrheic piglets in Korea. J. Vet. Diagn. Invest. 11:146-151 https://doi.org/10.1177/104063879901100207
  16. Moon, H. W., A. Robert, B. S. Schneider and L. Steve. 1986. Comparative prevalence of four enterotoxins genes among Escherichia coli isolated from swine. Am. J. Vet. Res. 47:210-212
  17. Nagy, B., T. A. Casey and H. W. Moon. 1990. Phenotype and Genotype of Escherichia coli isolated from pigs with postweaning diarrhea in hungry. J. Clin. Microbiol. 28:651-653
  18. Natro, J. P. and J. B. Kaper. 1998. Diarrheagenic Escherchia coli. Clin. Microbiol. Rev. 11:142-201
  19. Ojeniyi, B., P. Ahrens and A. Meyling. 1994. Detection of fimbrial and toxin genes in and their prevalence in piglets with diarrhea. The applfication of colony hybridization assay, polymerase chain reaction and phenotype assays. J. Vet. Med. B 41:49-59 https://doi.org/10.1111/j.1439-0450.1994.tb00205.x
  20. Osek, J. 1999. Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet. Microbiol. 68:209-217 https://doi.org/10.1016/S0378-1135(99)00109-1
  21. Soderlind, O., B. Thafvelin and R. Mollby. 1988. Virulence factors in Escherichia coli strains isolate from Swedish piglets with diarrhea. J. Clin. Microbiol. 26:879-884
  22. Spreeuwenberg, M. A. M., J. M. A. J. Verdonk, H. R. Gaskins and M. W. A. Verstegen. 2001. Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J. Nutr. 131:1520-1527
  23. Wilson, R. A. and D. H. Francis. 1986. Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. Am. J. Vet. Res. 2:213-217

Cited by

  1. Expression optimization of subunit fasG of 987P fimbriae in enterotoxigenic Escherichia coli (ETEC) vol.6, pp.01, 2009, https://doi.org/10.1017/S1479236209002551
  2. The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4–LMLN region vol.22, pp.1-2, 2011, https://doi.org/10.1007/s00335-010-9305-3
  3. Cloning and Expression of Genes Encoding F107-C and K88-1NT Fimbrial Proteins of Enterotoxigenic Escherichia coli from Piglets vol.53, pp.4, 2013, https://doi.org/10.1007/s12088-013-0386-z
  4. Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coliand their fimbriae vol.15, pp.1, 2014, https://doi.org/10.1186/s12863-014-0103-x
  5. Refined Candidate Region for F4ab/ac Enterotoxigenic Escherichia coli Susceptibility Situated Proximal to MUC13 in Pigs vol.9, pp.8, 2014, https://doi.org/10.1371/journal.pone.0105013
  6. F4+ ETEC infection and oral immunization with F4 fimbriae elicits an IL-17-dominated immune response vol.46, pp.1, 2015, https://doi.org/10.1186/s13567-015-0264-2
  7. Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets vol.21, pp.4, 2006, https://doi.org/10.5713/ajas.2008.70464
  8. Comparative evaluation of a vaccine candidate expressing enterotoxigenic Escherichia coli (ETEC) adhesins for colibacillosis with a commercial vaccine using a pig model vol.30, pp.26, 2006, https://doi.org/10.1016/j.vaccine.2012.03.087